The demise of retinal ganglion cells (RGCs) is characteristic of diseases of the retina such
as glaucoma and diabetic or ischemic retinopathies. Pigment epithelium-derived factor (PEDF) is a
multifunctional secreted protein that mediates neuroprotection and inhibition of angiogenesis in the
retina. We have studied expression and regulation of two of several receptors for PEDF, patatin-like
phospholipase 2 gene product/PEDF-R and laminin receptor (LR), in serum-starved RGC under
normoxia and hypoxia and investigated their involvement in the survival of retinal neuronal cells.
We show that PEDF-R and LR are co-expressed in RGC and R28 retinal precursor cells. Expression
of both receptors was enhanced in the presence of complex secretions from retinal glial (Müller)
cells and upregulated by VEGF and under hypoxic conditions. PEDF-R- and LR-knocked-down
cells demonstrated a markedly attenuated expression of anti-apoptotic Bcl-2 family members (Bcl-2,
Bcl-xL) and neuroprotective mediators (PEDF, VEGF, BDNF) suggesting that both PEDF-R and LR
mediate pro-survival effects of PEDF on RGC. While this study does not provide evidence for a
differential survival-promoting influence of either PEDF-R or LR, it nevertheless highlights the
importance of both PEDF receptors for the viability of retinal neurons.
| Identifer | oai:union.ndltd.org:DRESDEN/oai:qucosa:de:qucosa:88928 |
| Date | 10 January 2024 |
| Creators | Bürger, Susanne, Meng, Jie, Zwanzig, Annette, Beck, Mike, Pankonin, Maik, Wiedemann, Peter, Eichler, Wolfram, Unterlauft, Jan Darius |
| Publisher | MDPI |
| Source Sets | Hochschulschriftenserver (HSSS) der SLUB Dresden |
| Language | English |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, doc-type:article, info:eu-repo/semantics/article, doc-type:Text |
| Rights | info:eu-repo/semantics/openAccess |
| Relation | 369, 10.3390/ijms22010369 |
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