The lateral division of the central amygdala (CeAL) is a key structure at the limbic-motor interface regulating stress-responses and emotional learning. Endocannabinoid (eCB) signaling is heavily implicated in the regulation of stress-response physiology and emotional learning processes, however, the role of eCBs in the modulation of synaptic efficacy in the CeAL is not well understood. Here we describe the subcellular localization of type 1 cannabinoid receptors (CB1) and the eCB synthetic machinery at glutamatergic synapses in the CeAL and find that CeAL neurons exhibit multiple mechanistically and temporally distinct modes of postsynaptic eCB mobilization. Furthermore, this work demonstrates that following chronic stress exposure, eCB-mediated suppression of CeAL excitatory inputs is enhanced. Collectively, these data identify a prominent role for eCBs in the modulation of excitatory drive to CeAL neurons and provide insight into the mechanisms by which eCB signaling and exogenous cannabinoids could regulate stress-responses and emotional learning.
| Identifer | oai:union.ndltd.org:VANDERBILT/oai:VANDERBILTETD:etd-11242014-223852 |
| Date | 08 December 2014 |
| Creators | Ramikie, Teniel Sonya |
| Contributors | Sachin Patel MD., PhD., Karoly Mirnics, MD., PhD., Roger Cone, PhD., Sean Davies, PhD., Danny Winder, PhD. |
| Publisher | VANDERBILT |
| Source Sets | Vanderbilt University Theses |
| Language | English |
| Detected Language | English |
| Type | text |
| Format | application/pdf |
| Source | http://etd.library.vanderbilt.edu/available/etd-11242014-223852/ |
| Rights | restrictone, I hereby certify that, if appropriate, I have obtained and attached hereto a written permission statement from the owner(s) of each third party copyrighted matter to be included in my thesis, dissertation, or project report, allowing distribution as specified below. I certify that the version I submitted is the same as that approved by my advisory committee. I hereby grant to Vanderbilt University or its agents the non-exclusive license to archive and make accessible, under the conditions specified below, my thesis, dissertation, or project report in whole or in part in all forms of media, now or hereafter known. I retain all other ownership rights to the copyright of the thesis, dissertation or project report. I also retain the right to use in future works (such as articles or books) all or part of this thesis, dissertation, or project report. |
Page generated in 0.0017 seconds