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Vliv probiotik na imunologické vlastnosti buněk obranného systému

This disertation thesis aims to determine the effect of probiotic on the cells of immune system – human, procine and rat neutrophils and macrophages. Immune cells were exposed to 3 probiotics – Bifidobacterium bifidum, Lactobacillus rhamnosus and Enterococcus faecium, which are normally included in GIT. The first aim of this study was choose the most appropriate method for the isolation of human and porcine neutrophils and PBMC from peripheral blood. Other aims were to find out whether interactions of probiotics with neutrophils and macrophages affect their lifetime and lead to their structural changes. And whether intraperitoneal administration of probiotics lead to pro-inflammatory polarizoation of macrophages and affect viability of intraperitoneal immune cells. Structural changes of cells were detected by light and transmission electron microscopy. For detection of early and late apoptosis was used flow cytometry and for detection od cytolysis was used LDH aktivity. To determine of effect of probiotics on polarization of rat macrophages, qRT PCR was used. The results of this thesis suggest that all tested probiotics led to a significant increase in early apoptosis in porcine neutrophils and late apoptosis and cytolysis (LDH) in human neutrophils in vitro.

Identiferoai:union.ndltd.org:nusl.cz/oai:invenio.nusl.cz:425304
Date January 2018
CreatorsŠustrová, Tereza
Source SetsCzech ETDs
LanguageCzech
Detected LanguageEnglish
Typeinfo:eu-repo/semantics/doctoralThesis
Rightsinfo:eu-repo/semantics/restrictedAccess

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