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Previous issue date: 2015-12-09 / Aging is a complex and multifaceted process that includes physiological,
molecular and genetic changes that lead to functional decline of tissues and
organs, leading to increased susceptibility to disease and consequent increase
of mortality rates. In this context, chronic non-communicable diseases (NCDs)
are often found in the elderly, especially the onco-hematologic diseases,
including chronic lymphocytic leukemia (CLL). Considered as essentially a
disease of the elderly, CLL is a disease of clonal lymphoid infiltration of B cells
found in the bone marrow (BM), peripheral blood and lymph nodes, and
characterized as the most common type of leukemia in Western countries. LLC
expresses high levels of anti-apoptotic proteins, and decreased levels of proapoptotic
proteins, which explains the exaggerated increase of mature B
lymphocytes. In addition to changes protein levels, deregulation of microRNAs
(miRNAs) have been associated with the LLC. Numerous miRNAs are
associated with susceptibility to cancer, suggesting an important role in
carcinogenesis. In addition, several miRNAs are also involved in the regulation
pathways related to cell senescence and have effects on cell cycle progression,
suggesting that miRNAs can be considered good biomarkers in the senescence
process. The aim of the study was to evaluate the expression of miRNAs miR-
1248, miR-181a and miR-151a-3p by qPCR in patients with LLC and compare
with the elderly group without the disease. The expression of miR-181a was
significantly reduced in patients with CLL compared to individuals in the group
without LLC. However, the study showed no difference in the expression of
miRNAs in the groups with respect to age and sex. It is possible to suggest that
these miRNAs can act as regulators likely in aging, however, future studies will
determine the mechanism of expression of the same, and to discuss how best
to use them as diagnostic and therapeutic tools for aging and age-related
diseases such as CLL. / O envelhecimento ?? um processo complexo e multifatorial que inclui altera????es
fisiol??gicas, moleculares e gen??ticas que levam ao decl??nio funcional de tecidos
e ??rg??os, provocando o aumento da suscetibilidade ??s doen??as e consequente
eleva????o das taxas de mortalidade. Nesse contexto, as doen??as cr??nicas n??o
transmiss??veis (DCNT) s??o frequentemente encontradas em idosos,
principalmente as doen??as onco-hematol??gicas, dentre elas a leucemia
linfoc??tica cr??nica (LLC). Considerada essencialmente como uma doen??a de
idosos, a LLC ?? uma patologia linf??ide de infiltra????o clonal de c??lulas B
encontradas na medula ??ssea (MO), no sangue perif??rico e nos linfonodos,
sendo caracterizada como o tipo mais comum de leucemia em pa??ses
ocidentais. A LLC expressa n??veis elevados de prote??nas anti-apopt??ticas e
n??veis diminu??dos de prote??nas pr??-apopt??ticas, o que explica o aumento
exacerbado de linf??citos B maduros. Al??m das altera????es dos n??veis de
prote??nas, a desregula????o de microRNAs (miRNAs) tem sido associada ?? LLC.
In??meros miRNAs est??o associados ?? suscetibilidade ao c??ncer, sugerindo um
importante papel na carcinog??nese. Al??m disso, v??rios miRNAs tamb??m est??o
envolvidos na regula????o de vias relacionadas a senesc??ncia celular e t??m
efeitos sobre a progress??o do ciclo celular, sugerindo que os miRNAs podem
ser considerados bons biomarcadores no processo de senesc??ncia. O objetivo
do estudo foi avaliar a express??o dos miRNAs miR-1248, miR-181a e miR-
151a-3p por meio da qPCR em portadores de LLC e comparar com o grupo de
idosos sem a doen??a. A express??o do miR-181a foi significativamente reduzida
nos pacientes com LLC quando comparada aos indiv??duos do grupo sem LLC.
No entanto, o estudo demonstrou que n??o houve diferen??a na express??o dos
miRNAs nos grupos em rela????o ?? idade e ao sexo. ?? poss??vel sugerir que
esses miRNAs possam atuar como prov??veis reguladores no envelhecimento,
por??m, estudos futuros poder??o determinar o mecanismo da express??o dos
mesmos, bem como discutir a melhor forma de utiliz??-los como ferramentas
diagn??sticas e terap??uticas para o envelhecimento e doen??as relacionadas ??
idade, como por exemplo a LLC.
| Identifer | oai:union.ndltd.org:IBICT/oai:bdtd.ucb.br:tede/2072 |
| Date | 09 December 2015 |
| Creators | Longatti, Simone Cruz |
| Contributors | Vila??a, Karla Helena Coelho, Mascarenhas, Cintia do Couto |
| Publisher | Universidade Cat??lica de Bras??lia, Programa Strictu Sensu em Gerontologia, UCB, Brasil, Escola de Sa??de e Medicina |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/masterThesis |
| Format | application/pdf |
| Source | reponame:Biblioteca Digital de Teses e Dissertações da UCB, instname:Universidade Católica de Brasília, instacron:UCB |
| Rights | info:eu-repo/semantics/openAccess |
| Relation | -5510321348765290009, 500, 500, 600, 3870802503144686128, -969369452308786627 |
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