The effect of acetylene (C(,2)H(,2)) on nitrifying microorganisms and the production of nitrous oxide (N(,2)O) by the ammonia-oxidizing chemoautotroph Nitrosomonas europaea was measured in culture medium and in sterilized soil. / Acetylene strongly inhibited (K(,i) 0.66 Pa) the oxidation of NH(,4)('+) to hydroxylamine (NH(,2)OH) but not of NH(,2)OH to nitrite (NO(,2)('-)) by N. europaea. The oxidation of NH(,4)('+) to NH(,2)OH and NO(,3)('-) by the heterotrophic nitrifier, Arthrobacter sp. was not affected by C(,2)H(,2) (10 kPa). / Observed patterns of N(,2)O production by N. europaea in the presence of various combinations of NH(,4)('+), NO(,2)('-), C(,2)H(,2) and oxygen (O(,2)) supported earlier published suggestions that N(,2)O may be formed via an oxidative route from NH(,4)('+) and (or) NH(,2)OH, or via a reductive route from NO(,2)('-). The former process was O(,2)-dependent and inhibited by C(,2)H(,2), while the latter process was inhibited by O(,2) but not by C(,2)H(,2). / Sodium chlorate (NaClO(,3), 10 mM) inhibited the oxidation of both NH(,4)('+) and NO(,2)('-) in dual axenic suspensions of N. europaea and Nitrobacter winogradskyi. N. winogradskyi reduced ClO(,3)('-) to ClO(,2)('-) under both aerobic and anaerobic conditions. Evidence is presented which suggests that NH(,4)('+) oxidation is inhibited by the ClO(,2)('-) so produced.
| Identifer | oai:union.ndltd.org:LACETR/oai:collectionscanada.gc.ca:QMM.71847 |
| Date | January 1983 |
| Creators | Hynes, Russell K. (Russell Kenneth) |
| Publisher | McGill University |
| Source Sets | Library and Archives Canada ETDs Repository / Centre d'archives des thèses électroniques de Bibliothèque et Archives Canada |
| Language | English |
| Detected Language | English |
| Type | Electronic Thesis or Dissertation |
| Format | application/pdf |
| Coverage | Doctor of Philosophy (Department of Microbiology.) |
| Rights | All items in eScholarship@McGill are protected by copyright with all rights reserved unless otherwise indicated. |
| Relation | alephsysno: 000155557, proquestno: AAINK64585, Theses scanned by UMI/ProQuest. |
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