This study investigated possible reasons for the failure of the acetylene inhibition assay of denitrification in highly reducing environments and resulted in the isolation of a denitrifying Cytophaga from soil enriched by anaerobic incubation with glucose, nitrous oxide (N(,2)O), acetylene (C(,2)H(,2)), and sulfide (S('2-)). Such soil enrichments and pure cultures of the isolated Cytophaga (Is-11) reduced N(,2)O rapidly even in the presence of a normally inhibitory concentration of C(,2)H(,2) (4 kPa), providing S('2-) was present 8.0 umol (g soil)('-1) or 0.4 umol (mL culture)('-1) . The observed reaction appears to be unique to this soil Cytophaga since other organisms tested, using the same or similar experimental conditions, failed to show this response. / The isolate was characterized, after extensive comparative studies with five Cytophaga johnsonae strains, as a pigmented, Gram-negative, non-motile, gliding filamentous bacillus. Although these features established a taxonomic link with the family Cytophagaceae, the inability of Is-11 to digest chitin and DNA base composition of about 42.5 mol% (G+C) make the organism's specific affiliation uncertain.
| Identifer | oai:union.ndltd.org:LACETR/oai:collectionscanada.gc.ca:QMM.72068 |
| Date | January 1985 |
| Creators | Adkins, Anne M. |
| Publisher | McGill University |
| Source Sets | Library and Archives Canada ETDs Repository / Centre d'archives des thèses électroniques de Bibliothèque et Archives Canada |
| Language | English |
| Detected Language | English |
| Type | Electronic Thesis or Dissertation |
| Format | application/pdf |
| Coverage | Doctor of Philosophy (Department of Microbiology.) |
| Rights | All items in eScholarship@McGill are protected by copyright with all rights reserved unless otherwise indicated. |
| Relation | alephsysno: 000230950, proquestno: AAINL24071, Theses scanned by UMI/ProQuest. |
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