This study is entitled, “determination of the presence of antibiotics and bacterial pathogens with their susceptibility in fresh milk from the San Nicolas community and the cities of Penipe, Guano, and Chambo in the Chimborazo province”. It was funded by the Benson Institute of Brigham Young University and Nestle. The objectives were: 1) to determine the presence of antibiotics in fresh milk from the San Nicolas community and the cities of Penipe, Guano, and Chambo, 2) to determine the presence of bacterial pathogens in milk from the places previously mentioned, and 3) to detect the susceptibility of the isolated bacteria to the most commonly used antibiotics on the market. With regard to the presence of antibiotics, they were only found once in Chambo (beta-lactams) and once in San Nicolas (tetracycline). Three types of pathogenic bacteria were found: Escherichia coli, Streptococcus agalactiae, and Staphylococcus aureus. Only one bacterium was found that has not been studied: Pseudomonas aureoqinosa. This bacterium was found in milk from San Nicolas. With regard to the susceptibility of the bacteria to antibiotics, it was found that Escherichia coli was sensitive to the following antibiotics: nitrofurans, erimethoprina, trimethoprim and sulfonamides, and tetracyclines and spectomicina. Escherichia coli also showed medium sensitivity to chloramphenicol as well as resistance to ampicillin and sulfonamides. Streptococcus agalactiae was sensitive to lincomycin, neomycin, enrofloxacin, and clindamycin. It also showed medium sensitivity to erythromycin and low tetracycline and penicillin as well as resistance to gentamicin. Lastly, Staphylococcus aureus was sensitive to neomycin and enrofloxacin. In addition, it showed medium sensitivity to lincomycin, gentamicin, clindamycin, and tetracycline as well as resistance to low erythromycin and penicillin. This study was done over a four month period with at least 12 samples being collected from each area. A total of 60 samples were analyzed. Based on the results obtained, our recommendation is that hygienic measures are taken in obtaining and transporting milk to eliminate the growth of bacterial pathogens. Also, we recommend the use of the effective antibiotics listed above in order to completely eradicate these microorganisms.
| Identifer | oai:union.ndltd.org:BGMYU2/oai:scholarsarchive.byu.edu:etd-6396 |
| Date | 01 January 1997 |
| Creators | Marín, Lupe |
| Publisher | BYU ScholarsArchive |
| Source Sets | Brigham Young University |
| Detected Language | English |
| Type | text |
| Format | application/pdf |
| Source | Theses and Dissertations |
| Coverage | Riobamba (Ecuador), Chimborazo (Ecuador) |
| Rights | http://lib.byu.edu/about/copyright/ |
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