Indiana University-Purdue University Indianapolis (IUPUI) / Non-invasive brain stimulation is valuable for studying neural circuits and
treating various neurological disorders in human. However, current technologies of noninvasive
brain stimulation usually have low spatial and temporal precision and poor brain
penetration, which greatly limit their application. A new class of nanoparticles known as
magneto-electric nanoparticles (MENs) is highly efficient in coupling an externally
applied magnetics wave with generating local electric fields for neuronal activity
modulation. Here, a new type of MENs was developed that consisted of CoFe2O4-
BaTiO3 and had excellent magneto-electrical coupling properties. Calcium imaging
technique was used to demonstrate their efficacy in evoking neuronal activity in
organotyic and acute cortical slices that expressed GCaMP6 protein. For in vivo noninvasive
delivery of MENs to brain, fluorescently labeled MENs were intravenously
injected and attracted to pass through blood brain barrier to a targeted brain region by
applying a focal magnet field. Magnetic wave (~450 G at 10 Hz) applied to mouse brain
was able to activate cortical network activity, as revealed by in vivo two-photon and
mesoscopic imaging of calcium signals at both cellular and global network levels. The
effect was further confirmed by the increased number of c-Fos expressing cells after
magnetic stimulation. Histological analysis indicated that neither brain delivery of MENs
nor the subsequent magnetic stimulation caused any significant increases in the numbers
of GFAP and IBA1 positive astrocytes and microglia in the brain. MENs stimulation also
show high efficacy in short-term pain relieve when tested with a tibial nerve injury mouse
model. The study demonstrates the feasibility of using MENs as a novel efficient and
non-invasive technique of brain stimulation, which may have great potential for
translation.
| Identifer | oai:union.ndltd.org:IUPUI/oai:scholarworks.iupui.edu:1805/24094 |
| Date | 09 1900 |
| Creators | Nguyen, Tyler |
| Contributors | White, Fletcher, Blesch, Armin, Jin, Xiaoming, Rodgers, Richard, Khizroev, Sakhrat |
| Source Sets | Indiana University-Purdue University Indianapolis |
| Language | en_US |
| Detected Language | English |
| Type | Dissertation |
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