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Molekulární charakteristika transformantů \kur{L. esculentum} po vnesení genu pro manóza-6-fosfátizomerázu / The molecular analysis of transgenic \kur{Lycopersicon esculentum} plants harbouring incorporated pmi gene for phosphomannose isomerase

The aim of this study was to analyze number of incorporated T-DNA harbouring pmi and nptII transgenes and expression of the pmi gene in tomato transformants using Southern blot and Northern blot methods. The presence of a functional PMI protein was assesed using PMI-assay with Chlorophenol red dye. Mannose selection of tomato seeds was established and segregation patterns in T1 progeny were studied.

Identiferoai:union.ndltd.org:nusl.cz/oai:invenio.nusl.cz:47079
Date January 2007
CreatorsPŘIKRYLOVÁ, Pavla
Source SetsCzech ETDs
LanguageCzech
Detected LanguageEnglish
Typeinfo:eu-repo/semantics/masterThesis
Rightsinfo:eu-repo/semantics/restrictedAccess

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