The NF-κB/Rel family of transcription factors and IκB inhibitors play a key role in regulation of gene expression in inflammation and immunity. Previous studies from our laboratory suggested that steady-state levels of p65 and other NF-κB components in the normal mouse aorta determine the magnitude of NF-κB target gene expression in response to pro-inflammatory stimuli, however, the mechanism(s) by which steady-state levels of NF-κB components are set is not clear. This study aims at elucidating the mechanisms behind NF-κB homeostasis and how that affects atherosclerosis susceptibility. In HeLa cells and HUVEC, siRNA silencing of p65 correlated with reduced steady-state expression of a subset of NF-κB/Rel and IκB genes at the transcriptional and post-transcriptional levels, respectively, in addition to reducing TNFα-induced NF-κB/Rel and IκB gene expression. This correlation was also observed in atherosclerosis-susceptible mouse aortic endothelium suggesting the role of p65 in modulating NF-κB homeostasis and affecting atherosclerosis susceptibility.
| Identifer | oai:union.ndltd.org:LACETR/oai:collectionscanada.gc.ca:OTU.1807/31629 |
| Date | 04 January 2012 |
| Creators | Wasal, Karanvir |
| Contributors | Jongstra-Bilen, Jenny, Cybulsky, Myron |
| Source Sets | Library and Archives Canada ETDs Repository / Centre d'archives des thèses électroniques de Bibliothèque et Archives Canada |
| Language | en_ca |
| Detected Language | English |
| Type | Thesis |
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