HMB intake during Resistance Training (RT) is known to be an effective strategy to improve muscle mass & function and decrease FM. PURPOSE: To determine whether HMB administration during 10-wk RT would facilitate RT-induced improvement in neuromuscular functions, body composition, myofiber dimension and regenerative capacity in aged female rats. METHODS: After four animals were sacrificed for baseline muscle samples, twelve 19-month old female Sprague-Dawley rats (N=16) were randomly divided into two groups, HMB (0.46 g/kg/d, similar to 6 g/d in humans) and non-HMB. Both groups were trained every 3 day for 10 wks on ladder climbing exercise with weights attached to the tail. The MW, GS, inclined plane test were used to measure neuromuscular functions. Body composition was assessed by DXA before and after RT. DTI determined water diffusion in myofibers by calculating FA and eigenvalues. Muscle mRNA expressions of the regulators of myogenesis, mitogenesis, and cell cycle inhibition were analyzed using RT-PCR. The number of satellite cells and myonuclei associated with muscle regeneration capacity were analyzed using Pax7-stained stem-cell immunohistochemistry. RESULTS: There were significant (p 0.05). Although a main group effect for soleus myogenin mRNA approached significance (p=0.07), post hoc analysis showed significant increases in both HMB (+32%) and non-HMB (+40%) (p < 0.05). Soleus SC significantly increased in both HMB (+108%) and non-HMB (+100%) (p < 0.05) with no significant between group differences. Soleus MN also increased in both HMB (+32.2%) and non-HMB (+31.8%) (p < 0.05) with no significant between group differences. RSC for soleus muscle significantly increased in both HMB (+51.5%) and non-HMB (+46.4%) (p < 0.05) with no significant between group differences. CONCLUSION: HMB did not to exert any additive improvements in all parameters during 10 wks of intensive RT in aged female rats. The excessive effects resulting from this intense RT protocol maybe far exceeded our expectations. Intriguingly, however, our findings indicated that HMB administration during RT can facilitate a reduction in fat mass. Thus, intense RT alone might serve as an effective strategy to promote body composition, functionality, and myofiber hypertrophy in aged skeletal muscle. / A Thesis Submitted to the Department of Nutrition, Food, and Exercise Sciences in Partial Fulfillment of the Requirements for the Degree of Master of
Science. / Summer Semester, 2010. / July 23, 2010. / HMB, Skeletal Muscle, Aging, Resistance Exercise / Includes bibliographical references. / Jeong-Su Kim, Professor Directing Thesis; Robert J. Contreras, Committee Member; Bahram H. Arjmandi, Committee Member.
| Identifer | oai:union.ndltd.org:fsu.edu/oai:fsu.digital.flvc.org:fsu_180467 |
| Contributors | Park, Young-Min (authoraut), Kim, Jeong-Su (professor directing thesis), Contreras, Robert J. (committee member), Arjmandi, Bahram H. (committee member), Department of Nutrition, Food, and Exercise Science (degree granting department), Florida State University (degree granting institution) |
| Publisher | Florida State University, Florida State University |
| Source Sets | Florida State University |
| Language | English, English |
| Detected Language | English |
| Type | Text, text |
| Format | 1 online resource, computer, application/pdf |
| Rights | This Item is protected by copyright and/or related rights. You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s). The copyright in theses and dissertations completed at Florida State University is held by the students who author them. |
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