Tempeh belongs to the fermented probiotic soybean products. Tempeh is a valuable nutritional foodstuff due to its rich source of protein, content of unsaturated fatty acids essential for human and high amount of antioxidants. The theoretical part describes nutritional composition of tempeh and soybeans. In the thesis are discussed positive and negative effects of soy on human health. In the thesis were followed up changes in the nutritional components caused by the fermentation process and the action of microorganisms. The experimental part deals with the identification of microorganisms, which are contained in three types of tempeh. Microorganisms were detected using method polymerase chain reaction. DNA was isolated from the products by use phenol extraction, using magnetic microparticles F-kol 77ox and magnetic nanoparticles F79/L3 PLL. As part of the nutritional analysis changes in individual nutrients and other substances in soybeans after tempeh fermentation have been studied. In the chosen products total content of essential nutrients – carbohydrates, lipids and proteins were analyzed. Amount of antioxidants such as phenolics and flavonoids were analysed by UV-VIS spectrophotometry. Determination of vitamin E content which was analysed by HPLC and determination presence of fatty acids which was determined by GC was incorporated to this thesis.
| Identifer | oai:union.ndltd.org:nusl.cz/oai:invenio.nusl.cz:376881 |
| Date | January 2018 |
| Creators | Sobotková, Markéta |
| Contributors | Vespalcová, Milena, Němcová, Andrea |
| Publisher | Vysoké učení technické v Brně. Fakulta chemická |
| Source Sets | Czech ETDs |
| Language | Czech |
| Detected Language | English |
| Type | info:eu-repo/semantics/masterThesis |
| Rights | info:eu-repo/semantics/restrictedAccess |
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