Abstract
In this paper we demonstrate that matrix-assisted laser desorption ionization/time-of-flight (MALDI-TOF) mass spectrometry can be used to rapidly detect occult blood in human feces. The water-soluble hemoglobins, which are the most predominant proteins found in blood, were used as protein biomarkers for the presence of occult blood. The proteins were best extracted from feces using a small amount of pure water and then separated from the feces through centrifugation. We found that saturated 4-hydroxy-£\-cyanocinnamic acid (£\-CHC) solution was the best MALDI matrix for hemoglobin analysis; this matrix was mixed with the aqueous solution extracted from the feces at a volumetric ratio of 1:1. Although the MALDI-TOF MS results obtained using real samples were consistent with those provided through conventional chemical analysis, the detection limit of occult blood (0.1¡V1 £gg/mg of feces) in feces when using MALDI MS was approximately 10¡V100 times lower than that of the conventional approach. In addition, materials that commonly interfere with the conventional chemical assaying of occult blood in feces¡Xsuch as foods containing animal blood¡Xdo not interfere with the detection of the hemoglobin biomarkers during MALDI-TOF MS analysis.
| Identifer | oai:union.ndltd.org:NSYSU/oai:NSYSU:etd-0704106-005438 |
| Date | 04 July 2006 |
| Creators | Wu, Chen-I |
| Contributors | none, none, none |
| Publisher | NSYSU |
| Source Sets | NSYSU Electronic Thesis and Dissertation Archive |
| Language | Cholon |
| Detected Language | English |
| Type | text |
| Format | application/pdf |
| Source | http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0704106-005438 |
| Rights | unrestricted, Copyright information available at source archive |
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