Nuclear factor-erythroid 2 (NF-E2) is a basic leucine zipper (bZIP) transcription factor that recognizes the DNA binding site [(T/C)GCTGA(G/C)TCA (T/C)] that contains a central AP-1 motif (underlined), but is distinct from AP-1 factors (Jun, Fos) due to the requirement of residues outside of the AP-1 core. NF-E2 is a heterodimer comprised of a cap'n'collar factor (p45) and a small Maf protein (MafF, G or K). It has been shown that NF-E2 promotes the opening of chromatin throughout the beta-globin loci, promoting gene expression and erythroid differentiation. Also, the p45 subunit is restricted to hematopoietic progenitor, erythroid, megakaryocytic and mast cells while the small Mafs are ubiquitously expressed. / I have used the erythroleukemia cell lines, MEL and CB3, as model systems to study the role of NF-E2. MEL cells can be induced to differentiate, while CB3 cells, which do not express p45 due to a Friend leukemia virus insertion in one allele and the loss of the other, express only minimal levels of alpha- and beta-globin mRNA upon induction.
| Identifer | oai:union.ndltd.org:LACETR/oai:collectionscanada.gc.ca:QMM.79053 |
| Date | January 2002 |
| Creators | Moore, Amy |
| Contributors | Blank, Volker (advisor) |
| Publisher | McGill University |
| Source Sets | Library and Archives Canada ETDs Repository / Centre d'archives des thèses électroniques de Bibliothèque et Archives Canada |
| Language | English |
| Detected Language | English |
| Type | Electronic Thesis or Dissertation |
| Format | application/pdf |
| Coverage | Master of Science (Division of Experimental Medicine.) |
| Rights | All items in eScholarship@McGill are protected by copyright with all rights reserved unless otherwise indicated. |
| Relation | alephsysno: 001975007, proquestno: AAIMQ88268, Theses scanned by UMI/ProQuest. |
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