Cytogenetic studies to identify chromosomal aberrations in ovarian tumors allow for a better understanding of tumor biology. Fifteen ovarian tumors (1 benign, 7 borderline, and 7 malignant tumors) and one normal control were successfully characterized using conventional cytogenetic methods. Among simple karyotypic changes, trisomy 10, not previously reported in ovarian cancer, was confirmed with fluorescence in situ hybridization (FISH). This latter may be considered as a single causative event or as one among many other chromosomal alterations, being both associated with ovarian tumorigenesis and tumor progression. Among complex karyotypic abnormalities, one cell one (OV90) was further characterized using combined GTG banding and fluorescence in situ hybridization (FISH) techniques with painting probes from all the chromosomes, and chromosome specific centromeric probes, and a 10p telomeric probe derived from a half-YACs. The short arm of one chromosome 3 was deleted and replaced by a homogeneous staining region (HSR) which was demonstrated to be originated from amplification of yet unidentified genes on chromosome 22. We also identified a complex chromosome rearrangement (CCR) involving chromosome 9, 10 and 17. A dicentric chromosome, dic(9;17)(p11;p11), and a derivative chromosome, der(10)del(10)(pter)t(10;17)(p15;p11.1p11.2 or p13) were demonstrated, with a deletion in the short arm of chromosome 9, and a partial deletion of chromosome 17p. A translocation of chromosome 10p telomere to chromosome 1p was also observed. In addition, partial trisomy of chromosome 13q14-qter was also characterized and considered to result from a duplication and a pericentric inversion. These chromosomal aberrations were. identified in all cells analyzed and in each cell culture despite long-term cell culture, repeated freezing and thawing. These abnormalities are thus probably responsible for tumorigenesis, tumor evolution or in vitro survival of cancer cells. Further characteriz
| Identifer | oai:union.ndltd.org:LACETR/oai:collectionscanada.gc.ca:QMM.27918 |
| Date | January 1997 |
| Creators | Wang, Jia-Chi, 1968- |
| Contributors | Eydoux, Patrice (advisor) |
| Publisher | McGill University |
| Source Sets | Library and Archives Canada ETDs Repository / Centre d'archives des thèses électroniques de Bibliothèque et Archives Canada |
| Language | English |
| Detected Language | English |
| Type | Electronic Thesis or Dissertation |
| Format | application/pdf |
| Coverage | Master of Science (Department of Pathology.) |
| Rights | All items in eScholarship@McGill are protected by copyright with all rights reserved unless otherwise indicated. |
| Relation | alephsysno: 001601799, proquestno: MQ37176, Theses scanned by UMI/ProQuest. |
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