Cellular adhesion interactions involved in invasion and migration are crucial to cancer metastasis. Neoplastic tissue explants were cultivated in three-dimensional collagen lattices and monitored for the detachment of coordinated locomoting tumor cell clusters. Blocking monoclonal antibody treatment (10ug/mL) against the $ beta sb1$ and $ alpha sb2 beta sb1$ integrin receptors on cluster surfaces allowed the functional evaluation of these receptors during metastatic invasion. Clusters in treated wells exhibited a loss in migratory persistence, reduction in cluster motility and a greater amount of "stops" during migration as compared to control wells for both anti-$ beta sb1$ and anti-$ alpha sb2 beta sb1$ respectively. These results suggest that $ beta sb1$ integrins, and more specifically, $ alpha sb2 beta sb1$ play a significant role in maintaining normal cell-cell and cell-matrix interactions during metastatic invasion. Preliminary investigations using the anti-cancer drug, Taxol, against clusters within collagen matrices revealed a significant decrease in the total distance traveled, average speed and persistence, implicating its efficiency against certain cancers. The use of 3-D collagen systems for cell communication studies on locomoting tumor cell clusters may reveal novel and potentially important mechanisms involved in metastatic invasion as well as facilitate future assessment of potential anti-neoplastic drugs.
| Identifer | oai:union.ndltd.org:LACETR/oai:collectionscanada.gc.ca:QMM.27382 |
| Date | January 1996 |
| Creators | Muradali, Sheri. |
| Contributors | Noble, P. B. (advisor) |
| Publisher | McGill University |
| Source Sets | Library and Archives Canada ETDs Repository / Centre d'archives des thèses électroniques de Bibliothèque et Archives Canada |
| Language | English |
| Detected Language | English |
| Type | Electronic Thesis or Dissertation |
| Format | application/pdf |
| Coverage | Master of Science (Faculty of Dentistry) |
| Rights | All items in eScholarship@McGill are protected by copyright with all rights reserved unless otherwise indicated. |
| Relation | alephsysno: 001548793, proquestno: MQ29757, Theses scanned by UMI/ProQuest. |
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