OV90, a spontaneously immortalized epithelial ovarian cancer (EOC) cell line, has been shown to contain a homogeneously staining region (HSR) originating from chromosome 22. To identify the amplified genes, differential gene expression was assessed using high-density oligonucleotide array (chip) technology. Genomic differential PCR and dot blot analysis showed that overexpression is consistent with gene amplification, as all sequences tested within the 1 Mb region of 22q11.21 are present in increased copy number. However, overexpression was not common, as only two of 69 EOC samples evaluated using the Hu6800 chip showed overexpression of a gene amplified in OV90, and three other EOC cell lines did not have increased expression in this region. This study tested the approach of chip technology to identify genes involved in gene amplification, in a rapid and reliable manner.
| Identifer | oai:union.ndltd.org:LACETR/oai:collectionscanada.gc.ca:QMM.78239 |
| Date | January 2002 |
| Creators | Arcand, Suzanna Lise |
| Contributors | Tonin, Patricia (advisor) |
| Publisher | McGill University |
| Source Sets | Library and Archives Canada ETDs Repository / Centre d'archives des thèses électroniques de Bibliothèque et Archives Canada |
| Language | English |
| Detected Language | English |
| Type | Electronic Thesis or Dissertation |
| Format | application/pdf |
| Coverage | Master of Science (Department of Human Genetics.) |
| Rights | All items in eScholarship@McGill are protected by copyright with all rights reserved unless otherwise indicated. |
| Relation | alephsysno: 001974402, proquestno: AAIMQ88147, Theses scanned by UMI/ProQuest. |
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