Prostate carcinoma (CaP) is the most frequently diagnosed malignancy in men in Western countries. Increased activity of the androgen receptor (AR) and/or AR gene (AR) amplification in the majority of both androgen-dependent and androgen-independent prostate cancers suggest that the AR plays an important role in CaP. / The polymorphic AR CAGn, repeat correlates inversely with AR transactivation. To better understand the contribution of this repeat to CaP, we propose to replace the stable mouse AR CAG/CAA/CAC tract with various lengths of CAG, using gene knock-in methodology. The neo-NTR-HSV-tk cassette from the pPGKneoNTRtkpA vector was introduced into the ploxPneo-1 vector such that loxP sites flanked it. Various CAG repeats were then cloned into mouse AR genomic 5' end clone. Finally, the 5' and 3' mouse AR genomic DNA fragments were cloned into the targeting vector, upstream and downstream of the cassette with flanking loxP sites. Knock-in mice with various CAG repeat lengths will be generated using these constructs.
| Identifer | oai:union.ndltd.org:LACETR/oai:collectionscanada.gc.ca:QMM.84062 |
| Date | January 2005 |
| Creators | Mousavi, Gity |
| Publisher | McGill University |
| Source Sets | Library and Archives Canada ETDs Repository / Centre d'archives des thèses électroniques de Bibliothèque et Archives Canada |
| Language | English |
| Detected Language | English |
| Type | Electronic Thesis or Dissertation |
| Format | application/pdf |
| Coverage | Master of Science (Department of Human Genetics.) |
| Rights | All items in eScholarship@McGill are protected by copyright with all rights reserved unless otherwise indicated. |
| Relation | alephsysno: 002263186, proquestno: AAIMR22754, Theses scanned by UMI/ProQuest. |
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