ABSTRACT
GENETIC DIFFERENTIATION OF LIQUIDAMBAR ORIENTALIS MILL.
VARIETIES WITH RESPECT TO matK REGION OF CHLOROPLAST
GENOME
Ö / ZD& / #272 / LEK, Asli
M.S., Department of Biology
Supervisor: Prof. Dr. Zeki Kaya
August 2007, 87 pages
Liquidambar L. genus is represented with mainly 4 species in the world and one of
these species, Turkish sweet gum (Liquidambar orientalis Mill.) which is a relictendemic
species is naturally found in only southwestern Turkey, mainly in Mugla
Province. The limited distribution of species with two disputed varieties (var.
integriloba Fiori and var. orientalis) and increased anthropogenic threats to its
genetic resources signify the importance of studying genetic diversity in the species
to have better conservation and management programs. For this purpose, 18 different
populations were sampled throughout the species range and matK region of
chloroplast DNA (cpDNA) was sequenced to assess the genetic structure of the
species. Turkish Liquidambar orientalis populations were evaluated at two
categories: variety level and geographic level. Also, two sectors of matK region were
examined to assess which part of the region was more variable. All molecular
analysis was conducted in this study by using MEGA version 3.1 and Arlequin 2.000
softwares.
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Moleculer diversity analysis indicated that the population located in Fethiye-
Gü / nlü / kbasi district has the highest number of polymorphic sites. This population is
also genetically the most distant from the others (average genetic distance 0.0038).
Among the studied varieties, the average genetic distance within var. integriloba
(0.0016) which also includes population Fethiye-Gü / nlü / kbasi was the greatest.
Among the geographic regions, Mugla-1 including Fethiye-Kö / ycegiz-Aydin district
as well as population Fethiye-Gü / nlü / kbasi showed the highest average genetic
distances within the region with a value of 0.0015. According to the molecular
variance results, among varieties and among geographic regions, there was no
significant differentiation, but great amount of total variation was found (~86%)
within Turkish sweet gum populations. With respect to the Fst values among
varieties, the highest genetic differentiation was observed between var. orientalis and
unknown group (0.040). Furthermore, based on the results of phylogenetic analysis,
Turkish populations of L. orientalis have genetically closer to USA relative (L.
styraciflua L.) than Chinese relatives (L. acalycina H.T Chang and L. formosana
Hance).
In conclusion, 10 Turkish sweet gum populations were found to be important for
conservation issues. Furthermore, eight of these located in Mugla province and sixth
of them belong to var. integriloba. Especially Fethiye-Gü / nlü / kbasi, MarmarisÇ / etibeli
and Mugla-Kiyra populations should be included in either insitu or exsitu or
in both conservation programs in the future.
| Identifer | oai:union.ndltd.org:METU/oai:etd.lib.metu.edu.tr:http://etd.lib.metu.edu.tr/upload/3/12608807/index.pdf |
| Date | 01 September 2007 |
| Creators | Ozdilek, Asli |
| Contributors | Kaya, Zeki |
| Publisher | METU |
| Source Sets | Middle East Technical Univ. |
| Language | English |
| Detected Language | English |
| Type | M.S. Thesis |
| Format | text/pdf |
| Rights | To liberate the content for public access |
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