Natural strains of the Saccharomyces cerevisiae growing on solid médium form structured, biofilm -like colonies. This ability is depended on the surface adhesin Flo11p. The expression of the FLO11 gene is upregulated by the RNA-binding protein Whi3p, which is likely to have a negative effect on the level of the transcription factor Yap6p. The aim of this study was to determine whether Yap6p affects colony morphology and FLO11 expression. Analysis of FLO11 expression using the fluorescent proteins pFlo11-GFP and Flo11p-DsRed in WHI3-deletion strains demonstrated a negative effect of Yap6p on FLO11 expression and confirmed changes in the effect of Yap6p on FLO11 expression in the presence of NaCl. In the strain overexpressing YAP6, the fluorescence values of pFlo11-GFP and Flo11p-DsRed were lower than in the strain with deletion of the YAP6 and in the presence of NaCl there was observed the largest increase in fluorescence. Although Yap6 protein is thought to have a negative effect on FLO11 expression under standard culture conditions, it seems to be responsible for a significant increase in FLO11 expression in the presence of mild osmotic stress. In WHI3-deletion strains, there was observed a significant increase in structuredness of colonies growing in the presence of NaCl. Analysis of structured...
| Identifer | oai:union.ndltd.org:nusl.cz/oai:invenio.nusl.cz:447470 |
| Date | January 2021 |
| Creators | Voloshin, Danila |
| Contributors | Schierová, Michaela, Heidingsfeld, Olga |
| Source Sets | Czech ETDs |
| Language | Czech |
| Detected Language | English |
| Type | info:eu-repo/semantics/masterThesis |
| Rights | info:eu-repo/semantics/restrictedAccess |
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