The effects of copper on growth and phosphorus (P) utilization were investigated in the marine chlorophyte Ulva fasciata Delile. Both the daily specific growth rate and tissue P contents decreased as increasing CuSO4 concentrations, while the contents of total tissue, intracellular and cell-wall Cu increased. Based on the relationship between daily specific growth rate and external CuSO4 concentrations, the upper limit of U. fasciata is 100 uM CuSO4. After 4-day exposure to varying CuSO4 concentrations, Pi uptake was inhibited. Analysis of P fraction in U. fasciata exposed to 100 uM CuSO4 shows that the Cu-induced decline in total tissue P contents is mainly due to a decrease in both soluble reactive P (i.e. Pi). Exposure to 100 uM of Cu caused the accumulation of total tissue Cu contents to a plateau and then rose again at day 3, and tissue P contents and daily specific growth rate decreased at day 4. IC50 (concentration of 50 % inhibition) of daily specific growth rate and tissue P contents are 9.8 and 37.8 uM of Cu concentration in the medium, respectively, and 0.76 and 1.44 mg¡Dg-1 DW for total tissue Cu contents, respectively. Overall, Cu causes Cu accumulation in intracellular space and cell wall and decrease of growth and P contents of U. fasciata partly via Pi uptake inhibition.
| Identifer | oai:union.ndltd.org:NSYSU/oai:NSYSU:etd-0730102-214743 |
| Date | 30 July 2002 |
| Creators | Huang, Ya-Ling |
| Contributors | Tse-Min Lee, Li-Jen Liao, Meng-Hsien Chen |
| Publisher | NSYSU |
| Source Sets | NSYSU Electronic Thesis and Dissertation Archive |
| Language | Cholon |
| Detected Language | English |
| Type | text |
| Format | application/pdf |
| Source | http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0730102-214743 |
| Rights | unrestricted, Copyright information available at source archive |
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