Thesis (MSc)--Stellenbosch University, 2005. / ENGLISH ABSTRACT: This study describes:
I. The cloning of the CVP 19 gene and construction of the intracellular expression
vector pPIC3.5K-CYP19.
II. The transformation of the yeast, Pichia pastoris with the constructed vector.
III. The expression ofP450arom in Pichia pastoris.
IV. The determination of enzyme activity and isolation of the protein from the Pichia
pastoris cells.
V. The expression of P450c 17 in Pichia pastoris.
VI. The determination of kinetic constants for the conversion of progesterone to
170H-progesterone and 160H-progesterone by P450c17. / AFRIKAANSE OPSOMMING: Hierdie studie beskryf:
I. Die klonering van die CVP 19 geen en die konstruksie van die intrasellulêre
uitdrukkingsplasmied, pPIC3.5K-CYPI9.
II. Die transformasie van die gis, Pichia pastoris, met die gekonstrueerde plasmied.
III. Die uitdrukking van aromatase in Pichia pastoris.
IV. Die bepaling van ensiemaktiwiteit en die isolering van die proteïen vanuit Pichia
pastoris.
V. Die uitdrukking van P450c17 in Pichia pastoris.
VI. Die bepaling van kinetiese konstantes vir die omsetting van progesteroon na
170H-progesteroon en 160H-progesteroon deur P450c17.
| Identifer | oai:union.ndltd.org:netd.ac.za/oai:union.ndltd.org:sun/oai:scholar.sun.ac.za:10019.1/50418 |
| Date | 12 1900 |
| Creators | Wepener, Ilse |
| Contributors | Swart, P., Swart, A. C., Stellenbosch University. Faculty of Science. Dept. of Biochemistry. |
| Publisher | Stellenbosch : Stellenbosch University |
| Source Sets | South African National ETD Portal |
| Language | en_ZA |
| Detected Language | English |
| Type | Thesis |
| Format | 93 p. : ill. (some col.) |
| Rights | Stellenbosch University |
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