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Production, purification et caracterisation d'hemolysines de Treponema hyodysenteriae

The conditions by which the production, in liquid media, of hemolytic activity by growing cells of T. hyodysenteriae and T. innocens are described. / From a ribonucleic acid added culture broth of T. hyodysenteriae, two hemolysins are shown in the supernatant. For each of them, a purification scheme allowing the obtention of an homogeneous hemolytic preparation by gel filtration and electrophoresis, is proposed. One hemolysin is associated with ribonucleic acid (HN) while the later is associated with bovine serum albumin (HP). They share some properties: their synthesis is blocked by chloramphenicol, they do not have proteolytic or phospholipasic activities, they are insensitive to oxygen and their activity does not require bivalent cations or is sensitive to EDTA's action; they are lytic to all red blood cell's type tested. They differ by the size of their apparent molecular weight, their mode of action, the stability of their activity to temperature and different pH and, their pattern of sensitivity to proteolytic enzymes. / The isolation of a non-hemolytic mutant strain of T. hyodysenteriae and its use in a ligated ileal loop model in rabbit has shown that there is a relation between the loss of the hemolytic activity and the loss of the enterotoxic activity.

Identiferoai:union.ndltd.org:LACETR/oai:collectionscanada.gc.ca:QMM.71862
Date January 1984
CreatorsPicard, Benoit
PublisherMcGill University
Source SetsLibrary and Archives Canada ETDs Repository / Centre d'archives des thèses électroniques de Bibliothèque et Archives Canada
LanguageFrench
Detected LanguageEnglish
TypeElectronic Thesis or Dissertation
Formatapplication/pdf
CoverageDoctor of Philosophy (Department of Microbiology.)
RightsAll items in eScholarship@McGill are protected by copyright with all rights reserved unless otherwise indicated.
Relationalephsysno: 000195416, proquestno: AAINK66581, Theses scanned by UMI/ProQuest.

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