Bifunctional alkylating agents that cause DNA cross-linking are implicated in the pathogenesis of myelodysplastic syndrome (MDS) and MDS related acute myeloid leukemia (MDR-AML). Therefore, characterizing hematopoietic cell responses to DNA cross-link damage may be relevant to elucidating the molecular pathogenesis of MDS and MDR-AML. To search for genes selectively involved in the cellular response to DNA cross-linking agents, HL-60 cells were treated with bifunctional cross-linking agents. In cDNA microarray gene expression profiles, S100P mRNA was selectively upregulated in bifunctional alkylating agent treated HL-60 cells but not in cells treated by monofunctional agents. This upregulation was confirmed by virtual Northern blot and real time PCR analysis and was both drug dose and time dependent. S100P protein was induced with kinetics similar to that of S100P mRNA. The upregulation of S100P by HN2 suggests that it may have a role in the cellular response to cross-link DNA damage and pathogenesis of MDS and AML.
| Identifer | oai:union.ndltd.org:VANDERBILT/oai:VANDERBILTETD:etd-09092005-163509 |
| Date | 09 September 2005 |
| Creators | jiang, fen |
| Contributors | Fritz Parl, mark koury, Greg Sephel, Walter Chazin, David Gailani, James Jacobberger |
| Publisher | VANDERBILT |
| Source Sets | Vanderbilt University Theses |
| Language | English |
| Detected Language | English |
| Type | text |
| Format | application/pdf |
| Source | http://etd.library.vanderbilt.edu/available/etd-09092005-163509/ |
| Rights | unrestricted, I hereby certify that, if appropriate, I have obtained and attached hereto a written permission statement from the owner(s) of each third party copyrighted matter to be included in my thesis, dissertation, or project report, allowing distribution as specified below. I certify that the version I submitted is the same as that approved by my advisory committee. I hereby grant to Vanderbilt University or its agents the non-exclusive license to archive and make accessible, under the conditions specified below, my thesis, dissertation, or project report in whole or in part in all forms of media, now or hereafter known. I retain all other ownership rights to the copyright of the thesis, dissertation or project report. I also retain the right to use in future works (such as articles or books) all or part of this thesis, dissertation, or project report. |
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