Characterization of fibrinogen-binding surface protein B (FbsB) in human blood coagulation and fibrinolysis, and of the NH2-terminal dipeptide of staphylocoagulase (SC) in conformational prothrombin activation are presented in this thesis. FbsB was hypothesized to bind fibrinogen and to be a member of the Zymogen Activator and Adhesion Protein (ZAAP) family. Kinetic assays, affinity and size-exclusion chromatography experiments, and Western blots performed in these studies determined that FbsB neither binds fibrinogen nor belongs to the ZAAP family. However, it was concluded that FbsB binds human plasmin and plasminogen, significantly inhibits the rate of plasmin substrate hydrolysis, and enhances plasminogen activation in the presence of tissue-type plasminogen activator. FbsB may be only the second characterized Streptococcus agalactiae protein capable of contributing to the spread of infection through its interaction with human fibrinolytic proteins. <p>
There remains a gap in knowledge about the importance of the sequence of the NH2-terminal dipeptide of SC in conformational prothrombin activation and the impact these conserved residues have in physiological serine protease activation. Kinetic assays with 29 different NH2-terminal SC(1-246) mutants revealed the relative promiscuity of these residues in prothrombin activation. Seven of the mutants screened activate prothrombin with 100-160% of the activity of wild-type. This data, along with future studies, may provide vital information about the mechanisms of prothrombin activation, and serine protease zymogen activation in general. <p>
| Identifer | oai:union.ndltd.org:VANDERBILT/oai:VANDERBILTETD:etd-07152011-200536 |
| Date | 26 July 2011 |
| Creators | Davis, Mary Elizabeth |
| Contributors | Paul E. Bock, Ph.D., Ingrid Verhamme, Ph.D., Richard Hoover, Ph.D. |
| Publisher | VANDERBILT |
| Source Sets | Vanderbilt University Theses |
| Language | English |
| Detected Language | English |
| Type | text |
| Format | application/pdf |
| Source | http://etd.library.vanderbilt.edu/available/etd-07152011-200536/ |
| Rights | restricted, I hereby certify that, if appropriate, I have obtained and attached hereto a written permission statement from the owner(s) of each third party copyrighted matter to be included in my thesis, dissertation, or project report, allowing distribution as specified below. I certify that the version I submitted is the same as that approved by my advisory committee. I hereby grant to Vanderbilt University or its agents the non-exclusive license to archive and make accessible, under the conditions specified below, my thesis, dissertation, or project report in whole or in part in all forms of media, now or hereafter known. I retain all other ownership rights to the copyright of the thesis, dissertation or project report. I also retain the right to use in future works (such as articles or books) all or part of this thesis, dissertation, or project report. |
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