Podocytes are highly differentiated glomerular epithelial cells that play an essential role in the establishment of the glomerular filtration barrier, a structural apparatus that selectively restricts the filtration of different macromolecules in the blood stream on the basis of their sizes, shape and charge. Podocyte dysfunction, one of the major causes of proteinuria, is of pathogenetic and prognostic significance in human glomerular disease. My study is focused on the investigation of novel pathways leading to podocyte dysfunction and proteinuria.
In the first part, immunoblotting and quantitative reverse transcriptase PCR (RT-PCR) were used to demonstrate that LIM and senescent cell antigen-like domains 1 (PINCH1) is induced and undergoes nuclear translocation in podocytes after transforming growth factor, beta 1 (TGF-beta1) treatment. Bioinformatics analysis revealed the putative nuclear export signal/nuclear localization signal (NES/NLS) at the PINCH1 C-terminus which is required for its nuclear translocation. Immunoprecipitation and GST pull-down assay identified the interaction between PINCH1 and Wilms tumor 1 (WT1) which led to suppression of the WT1-mediated podocalyxin gene expression. In vivo, PINCH1 also underwent nuclear translocation and interacted with WT1 after TGF-beta1 stimulation. Our data identifies nuclear transcription factor WT1 as a novel binding partner for PINCH1, and provides novel insight into the mechanism of podocyte dysfunction under pathological conditions.
In the second part, RT-PCR results revealed that treatment with TGF-beta1 induced gene expression of several wingless-type MMTV integration site family members (Wnts), predominantly Wnt1, and activated beta-catenin in mouse podocytes. Wnt antagonist Dickkopf-1 (DKK1) blocked TGF-beta1-induced beta-catenin activation and preserved nephrin expression. In vivo, ectopic expression of constitutively active TGF-beta1 induced Wnt1 expression, activated glomerular beta-catenin, upregulated its downstream target genes, and led to podocyte injury and proteinuria. Consistently, concomitant expression of DKK1 gene abolished beta-catenin activation in mouse glomeruli, inhibited TGF-beta1-triggered Wnt/beta-catenin target genes, and ameliorated proteinuria. These results establish a role for Wnt/beta-catenin signaling in the pathogenesis of podocyte injury and also suggest that this signaling pathway could be exploited as a therapeutic target for the treatment of proteinuric kidney diseases.
| Identifer | oai:union.ndltd.org:PITT/oai:PITTETD:etd-11292010-153331 |
| Date | 29 November 2010 |
| Creators | Wang, Dan |
| Contributors | Satdarshan P.S. Monga, Donna B. Stolz, Ora A. Weisz, Reza Zarnegar, Cary Wu, Youhua Liu |
| Publisher | University of Pittsburgh |
| Source Sets | University of Pittsburgh |
| Language | English |
| Detected Language | English |
| Type | text |
| Format | application/pdf |
| Source | http://etd.library.pitt.edu/ETD/available/etd-11292010-153331/ |
| Rights | restricted, I hereby certify that, if appropriate, I have obtained and attached hereto a written permission statement from the owner(s) of each third party copyrighted matter to be included in my thesis, dissertation, or project report, allowing distribution as specified below. I certify that the version I submitted is the same as that approved by my advisory committee. I hereby grant to University of Pittsburgh or its agents the non-exclusive license to archive and make accessible, under the conditions specified below, my thesis, dissertation, or project report in whole or in part in all forms of media, now or hereafter known. I retain all other ownership rights to the copyright of the thesis, dissertation or project report. I also retain the right to use in future works (such as articles or books) all or part of this thesis, dissertation, or project report. |
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