Peas (Pisum Sativum L. Var Alaska) were allowed to absorb calcium and magnesium chloride for 8 hours and then were germinated in a potassium phosphate buffer pH 6.5 for 40 hours. Peas were then treated with 0, 0.1, 0.3, or 0.5 ppm dicamba (3,6-dichloro-o-anisic acid) and harvested at 24, 48, and 72 hour intervals. The following determinations were recorded: Root elongation, nuclear volume, and anatomical modifications.
Root elongation recordings showed that dicamba prevented normal root elongation. Treatments induced very short thick primary roots.
Measurements of nuclear volume indicated that all herbicide concentrations were able to reduce the total volume. Several other compounds, (chloramphenicol, actinomycin D and colchicine), were introduced to better characterize the actions of dicamba. Chloramphenicol and colchicine were responsible for nuclear volume reductions.
Dicamba induced major anatomical alterations of treated pea roots. In the region 1 millimeter from the root tip, cortical cells were induced to divide more profusely. The diameter of treated roots exceeded that of untreated root tips.
In the region 3 millimeters from the root tip, excessive cellular division and swelling resulted in cortical damage.
| Identifer | oai:union.ndltd.org:UTAHS/oai:digitalcommons.usu.edu:etd-4509 |
| Date | 01 May 1974 |
| Creators | Ovard, Brent George |
| Publisher | DigitalCommons@USU |
| Source Sets | Utah State University |
| Detected Language | English |
| Type | text |
| Format | application/pdf |
| Source | All Graduate Theses and Dissertations |
| Rights | Copyright for this work is held by the author. Transmission or reproduction of materials protected by copyright beyond that allowed by fair use requires the written permission of the copyright owners. Works not in the public domain cannot be commercially exploited without permission of the copyright owner. Responsibility for any use rests exclusively with the user. For more information contact Andrew Wesolek (andrew.wesolek@usu.edu). |
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