<p>Ribose 5-phosphate isomerase (Rpi) is one of the major enzymes of the pentose phosphate pathway, where it catalyses the inter-conversion of ribose 5-phosphate (R5P) and ribulose 5-phosphate. Two forms of this isomerase with no significant amino acid sequence similarity exist, RpiA and RpiB. This thesis describes RpiB from the organisms <i>Mycobacterium tuberculosis</i> (<i>Mt</i>) and <i>Escherichia coli</i> (<i>Ec</i>) from a structural and functional point of view.</p><p>Since the <i>E. coli</i> genome encodes both an RpiA and an RpiB, which generally is not expressed, it has been proposed that <i>Ec</i>RpiB has a different role as an allose-6-phosphate isomerase. Activity measurements presented here show that <i>Ec</i>RpiB does have this second activity. </p><p>In the <i>M. tuberculosis</i> genome there is only a gene for RpiB. The crystal structure of <i>Mt</i>RpiB was solved in complex with several different inhibitors designed to mimic the reaction intermediate as well as with the substrate, R5P. The organisation of the active site in these structures could be used to derive the reaction mechanism for <i>Mt</i>RpiB and for other RpiBs in general. Activity measurements of <i>Mt</i>RpiB showed that it can catalyse the R5P isomerisation, but not the allose 6-phosphate reaction. Differences observed in the active site between <i>Ec</i>RpiB and <i>Mt</i>RpiB explain these kinetic results. </p><p>Activity measurements and a structure of an <i>Ec</i>RpiB mutant, where histidine99 was changed to asparagine, implies that RpiB catalyses the first step of the reaction in which the sugar ring must be opened, and gives a possible explanation for how this could occur. </p><p>Inhibition studies have uncovered a compound that selectively inhibits <i>Mt</i>RpiB over RpiA from spinach, which is homologous to the human RpiA. Differences in the inhibition patterns and active site residues of these two species’ Rpi may provide information for future virtual screening approaches, with the aim of discovering new anti-tuberculosis agents.</p>
| Identifer | oai:union.ndltd.org:UPSALLA/oai:DiVA.org:uu-8182 |
| Date | January 2007 |
| Creators | Roos, Annette K. |
| Publisher | Uppsala University, Department of Cell and Molecular Biology, Uppsala : Acta Universitatis Upsaliensis |
| Source Sets | DiVA Archive at Upsalla University |
| Language | English |
| Detected Language | English |
| Type | Doctoral thesis, comprehensive summary, text |
| Relation | Digital Comprehensive Summaries of Uppsala Dissertations from the Faculty of Science and Technology, 1651-6214 ; 332 |
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