Searches for sequence similarity against a previously isolated and known ABA regulated partial geranium cDNA on GenBank and TIGR databases gave results indicating sequence similarities to both firefly luciferase and 4-coumarate:CoA Ligase (4-CL). To determine whether or not this geranium cDNA, and a root mRNA from Arabidopsis that hybridized with the geranium cDNA, is 4-CL, specific Arabidopsis 4-CL primers were used for expression analysis by RT-PCR of Arabidopsis 4-CL mRNA from Arabidopsis root tissue. The expression pattern of the Arabidopsis 4-CL mRNA was identical with the expression pattern of the mRNA that hybridized with the geranium cDNA. This matching expression pattern gives very strong evidence that our geranium cDNA is 4-CL like and that Arabidopsis 4-CL is downregulated during water stress by ABA. This is significant in that it has not previously been reported. Northern analysis and PCR product sequence analysis further confirmed this expression data.
Identifer | oai:union.ndltd.org:ETSU/oai:dc.etsu.edu:etd-1089 |
Date | 01 December 2001 |
Creators | Ashley, David W |
Publisher | Digital Commons @ East Tennessee State University |
Source Sets | East Tennessee State University |
Detected Language | English |
Type | text |
Format | application/pdf |
Source | Electronic Theses and Dissertations |
Rights | Copyright by the authors. |
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