In the course of our efforts to resolve Pi transport mechanisms in the model fungus
Neurospora crassa we identified a null mutant, par-1, that displays enhanced Pi
transport activity specifically under alkaline growth conditions. The PAR-1 protein is
related to PalF of Aspergillus nidulans, which is one component of an ambient pH
signaling pathway that is conserved among fungi. A deletion mutant for the PacC
homolog, another component of the same pathway, phenocopies par-1, demonstrating
that a defect in pH signaling is responsible for the altered Pi transport activity. Our
results indicate that pH signaling in N. crassa plays an important role in coordinating
high and low affinity Pi transport in response to ambient pH, but through different
mechanisms. Sulfate acquisition also is influenced by pH signaling, suggesting that this
regulatory system has a broad role in nutrient uptake and homeostasis.
| Identifer | oai:union.ndltd.org:tamu.edu/oai:repository.tamu.edu:1969.1/4796 |
| Date | 25 April 2007 |
| Creators | Kennedy, Patrick Wade |
| Contributors | Versaw, Wayne K. |
| Publisher | Texas A&M University |
| Source Sets | Texas A and M University |
| Language | en_US |
| Detected Language | English |
| Type | Book, Thesis, Electronic Thesis, text |
| Format | 1545103 bytes, electronic, application/pdf, born digital |
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