The proteins required for catabolism of glycerol 3- phosphate are encoded by the genes of the <i>glp</i> regulon of Escherichia coli and are under negative transcriptional regulation by the <i>glpR</i>-encoded repressor. The <i>glpR</i> gene is adjacent to, and is transcribed divergently from, <i>glpD</i>. <i>GlpR</i> and <i>glpD</i> are separated by two open reading frames, designated <i>glpE</i> and <i>glpG</i>, encoding proteins of unknown function. The <i>glpD</i>-encoded aerobic sn-glycerol 3-phosphate dehydrogenase is a cytosplasmic membrane-associated respiratory enzyme. The nucleotide sequence of <i>glpD</i> was determined. An open reading frame of 501 codons was preceded by a consensus Shine-Dalgarno sequence. The proposed translational start and reading frame of glpD were confirmed by determining the nucleotide sequence across the fusion joint of a <i>glpD-lacZ</i>- translational fusion. / Ph. D.
Identifer | oai:union.ndltd.org:VTETD/oai:vtechworks.lib.vt.edu:10919/39955 |
Date | 19 October 2005 |
Creators | Austin, T. Denise |
Contributors | Biochemistry and Nutrition, Larson, Timothy J., Dean, Dennis R., Gregory, Eugene M., Potts, Mokahy, Sitz, Thomas O. |
Publisher | Virginia Tech |
Source Sets | Virginia Tech Theses and Dissertation |
Language | English |
Detected Language | English |
Type | Dissertation, Text |
Format | xii, 163 leaves, BTD, application/pdf, application/pdf |
Rights | In Copyright, http://rightsstatements.org/vocab/InC/1.0/ |
Relation | OCLC# 24119136, LD5655.V856_1991.A988.pdf |
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