Light is one of the major environmental factors that controls plant development, through
a process known as photomorphogenesis. Plants perceive light via photoreceptors, and
the information used to direct a myriad of developmental responses. Analysis of mutants
defective in photomorphogenic responses elucidates the complex interactions between
light and plants. Previous genetic screens have yielded a class of mutants which exhibit
exaggerated responses to ambient light, designated shl (seedling hyper-responsive to
light). The following work encompasses the identification of five new shl mutants, a
detailed examination of one of these mutants (shl7), and of the SHL7 gene. The mutants
were isolated in a low-white light screen of seedlings derived from T-DNA mutagenesis.
Each of the mutants exhibits a heritable hyper-responsive phenotype in low-white light,
but displays minimal effects in darkness. For each, a putative site of T-DNA insertion has
been located. In addition to a low-white light phenotype, the shl7 mutant exhibits a mild
hyper-responsive phenotype to 670 nm red and 735 nm far-red light, but significant
hyper-responses to 420 nm blue light. SHL7 encodes a small, unique, and previously
undescribed protein annotated as At4g04925. GFP protein fusion analysis indicates that
the protein is localized to mitochondria.
| Identifer | oai:union.ndltd.org:tamu.edu/oai:repository.tamu.edu:1969.1/ETD-TAMU-2008-08-66 |
| Date | 16 January 2010 |
| Creators | Grum, Daniel S. |
| Contributors | Pepper, Alan |
| Source Sets | Texas A and M University |
| Language | en_US |
| Detected Language | English |
| Type | Book, Thesis, Electronic Dissertation |
| Format | application/pdf |
Page generated in 0.0019 seconds