The skin forms an attractive and accessible route for systemic delivery of drugs as alternative
to other methods of administration, such as the oral and parental methods because of the
problems associated with last mentioned methods. The lipophilic character of the stratum
corneum, coupled with its intrinsic tortuosity, ensures that it almost always provides the
principal barrier to the entry of drug molecules into the skin.
Due to the fact that methotrexate (MTX) and acyclovir (ACV) have poor penetration
properties through the skin, the aim of this study was to enhance the permeation of
methotrexate and acyclovir with the use of two lamellar gel phase systems (LPGS)
(Physiogel® NT and Physiogel® Dermaquadrille) and with Emzaloid® as transdermal drug
delivery systems.
Three different sets of experiments were done in this study: 1) the viscosity of the two
Physiogel® creams was measured as an indication of stability and to determine whether the
internal structure of the Physiogel® creams were affected by the investigated drugs; 2) the
drug release rate from the three drug delivery vehicles was measured with a Vankel ®
dissolution apparatus; 3) in vitro permeation studies were preformed using vertical Franz
diffusion cells with human epidermal skin clamped between the donor and receptor
compartments. The skin was hydrated with PBS buffer for one hour before 1% mixtures of
the drugs in both the Physiogel® creams and Emzaloid® were applied to the donor chamber.
Samples were taken at 2, 4, 6, 8, 10, 12 and 24 hours. It was then analysed by HPLC for
methotrexate and acyclovir. The fluxes of drug permeation were determined.
The viscosity measurements confirmed that the internal structure of the two Physiogel®
creams was not influenced by the drugs. Acyclovir and methotrexate were both released from
the delivery vehicles. There was an enhancement of acyclovir through the skin from one of
the Physiogel® creams. The permeability of methotrexate in the presence of the two
Physiogel® vehicles was not significantly enhanced. Emzaloid® as delivery vehicle increased
the penetration of both drugs through the skin significantly.
The lamellar gel phase system mimics the structure of the stratum corneum, but does not
improve the drug permeation through the stratum corneum significantly. The utilisation of
Emzaloid® as a drug delivery system could be advocated from these findings. As could be
seen from the penetration profiles Emzaloid® was a superior delivery system for methotrexate
and acyclovir compared to the lamellar gel phase systems. / Thesis (M.Sc. (Pharmaceutics))--North-West University, Potchefstroom Campus, 2005.
| Identifer | oai:union.ndltd.org:netd.ac.za/oai:union.ndltd.org:nwu/oai:dspace.nwu.ac.za:10394/665 |
| Date | January 2004 |
| Creators | Reynecke, Sonique |
| Publisher | North-West University |
| Source Sets | South African National ETD Portal |
| Detected Language | English |
| Type | Thesis |
Page generated in 0.0021 seconds