A cDNA sequence representing a <i> Brassica carinata</i> gene the expression of which is induced by copper chloride treatment, was isolated from a library constructed with mRNA from treated leaves, and designated CIL1 (COPPER CHLORIDE INDUCED in LEAVES). A Basic Local Alignment Search Tool search revealed that CIL1 has similarities to an auxin-induced gene, AIR12 from <i>Arabidopsis thaliana</i>. Southern blot analysis of CIL1 in <i>B. carinata, B. nigra</i> and <i>B. oleracea</i> indicated that it is a member of a small multigene family. Antisense CIL1 transgenic plants were generated to investigate the function of CIL1, and the resulting transformants displayed increased secondary branching suggesting that CIL1 has a role in regulating hormone content or plant architecture. Results of induction studies indicate that the auxin analog a-naphthalene acetic acid, the cytokinin 6-benzylaminopurine, and +/- abscisic acid increase expression of CIL1. Seven CIL1 antisense lines were grown to the T4 generation and were confirmed homozygous. Analysis of CIL1 expression using real-time quantitative RT-PCR showed reduced expression in every examined line. Transgenic plants produced many leaves at the lateral meristems indicating a release of apical dominance. Additionally, the concentrations of auxins, cytokinins, and abscisic acid were altered in the roots and stems of transgenic plants compared to non-transformed plants. Therefore, CIL1 has a role in regulating hormone content that affects lateral meristem activity, apical dominance, and leaf production.
| Identifer | oai:union.ndltd.org:USASK/oai:usask.ca:etd-08222005-115050 |
| Date | 22 August 2005 |
| Creators | Gibson, Shawn |
| Contributors | Wilson, Kenneth E., Taylor, Janet, Sawhney, Vipen K., Bonham-Smith, Peta C. |
| Publisher | University of Saskatchewan |
| Source Sets | University of Saskatchewan Library |
| Language | English |
| Detected Language | English |
| Type | text |
| Format | application/pdf |
| Source | http://library.usask.ca/theses/available/etd-08222005-115050/ |
| Rights | unrestricted, I hereby certify that, if appropriate, I have obtained and attached hereto a written permission statement from the owner(s) of each third party copyrighted matter to be included in my thesis, dissertation, or project report, allowing distribution as specified below. I certify that the version I submitted is the same as that approved by my advisory committee. I hereby grant to University of Saskatchewan or its agents the non-exclusive license to archive and make accessible, under the conditions specified below, my thesis, dissertation, or project report in whole or in part in all forms of media, now or hereafter known. I retain all other ownership rights to the copyright of the thesis, dissertation or project report. I also retain the right to use in future works (such as articles or books) all or part of this thesis, dissertation, or project report. |
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