Membrane proteins specific to cold acclimation were studied in alfalfa (Medicago falcata L. cv Anik) seedlings and cell cultures. They were identified following separation on polyacrylamide gels and localized as far as possible to specific membranes by fractionation on continuous sucrose gradient and analysis of marker enzyme assays. / With cold acclimation, certain membrane proteins from seedlings showed subtle changes (mainly increases) in their steady-state amount and in their net synthesis rate. Most of these changes were in proteins with molecular weights below 100 kDa and were associated with light fractions of the sucrose gradient, favoring a Golgi, endoplasmic reticulum or tonoplast location for the proteins. Preliminary work done on membrane proteins from cell cultures showed more pronounced changes with cold acclimation. Most of the changes were in proteins with molecular weights below 100 kDa and were associated with heavy fractions of the sucrose gradient. Since they are easier to harvest and to manipulate, cell cultures appear to be the better system to use in future studies.
| Identifer | oai:union.ndltd.org:LACETR/oai:collectionscanada.gc.ca:QMM.56916 |
| Date | January 1992 |
| Creators | Bourassa, Hélène |
| Contributors | Poole, R. J. (advisor), Dhindsa, R. S. (advisor) |
| Publisher | McGill University |
| Source Sets | Library and Archives Canada ETDs Repository / Centre d'archives des thèses électroniques de Bibliothèque et Archives Canada |
| Language | English |
| Detected Language | English |
| Type | Electronic Thesis or Dissertation |
| Format | application/pdf |
| Coverage | Master of Science (Department of Biology.) |
| Rights | All items in eScholarship@McGill are protected by copyright with all rights reserved unless otherwise indicated. |
| Relation | alephsysno: 001327405, proquestno: AAIMM87625, Theses scanned by UMI/ProQuest. |
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