A 17.1 Kb genetic element encoding for mercury resistance (OCT-Hg^r) was shown to translocate from its original location on the OCT plasmid to the resistance plasmid, RPl, in Pseudomonas putida. Analysis of RPl-Hg^r recombinant plasmids revealed that insertion of mercury resistance genes into RPl could occur at a variety of sites, with all recombinants having common EcoRI restriction fragments of 9.4, 3.8, 2.3, and 1.6 Kb, derived from the insertion. Hybridization analysis suggested the existence of extensive homology between this insertion and the prototypic mercury resistance transposon, Tn501, as well as the location of a similar merA sequence. Although the overall size was shown to be quite different from Tn501, striking physical similarities are shared between these two elements.
| Identifer | oai:union.ndltd.org:unt.edu/info:ark/67531/metadc500381 |
| Date | 05 1900 |
| Creators | Armbruster, Steven C. (Steven Christopher) |
| Contributors | Kunz, Daniel A., Benjamin, Robert C., O'Donovan, Gerard A. |
| Publisher | University of North Texas |
| Source Sets | University of North Texas |
| Language | English |
| Detected Language | English |
| Type | Thesis or Dissertation |
| Format | vi, 49 leaves: ill., Text |
| Rights | Public, Armbruster, Steven C. (Steven Christopher), Copyright, Copyright is held by the author, unless otherwise noted. All rights reserved. |
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