The Master's thesis deals with the effect of feed enrichment with various types of oils on the deposition of fatty acids in selected tissues. Forty male Wistar Albino rats were divided into four groups and fed for 10 weeks daily ad libitum with a basic feed mixture enriched with 8 % palm, 8 % safflower, 8 % fish oil or 8 % microalgae Schizochytrium sp. oil. Rats had also free access to the drinking water. Fat from the tissue samples (liver, muscle, visceral fat) was extracted, converted to methyl esters and analyzed with usage of gas chromatography. The results of the experiment show that the addition of various types of oils to the feed of model organism can influence the deposition of fatty acids in its tissues. The addition of fish oil and microalgae Schizochytrium sp. oil caused statistically significant decrease (p < 0,05) in the ratio of PUFA n-6/n-3 in all observed tissues to a value of 1 or less compared to the addition of palm and safflower oil. Low ratio of n-6/n-3 polyunsaturated fatty acids is considered to be more desirable in reducing the risk of cardiovascular diseases.
| Identifer | oai:union.ndltd.org:nusl.cz/oai:invenio.nusl.cz:427721 |
| Date | January 2019 |
| Creators | Kaňoková, Linda |
| Source Sets | Czech ETDs |
| Language | Czech |
| Detected Language | English |
| Type | info:eu-repo/semantics/masterThesis |
| Rights | info:eu-repo/semantics/restrictedAccess |
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