In a minimal medium supplemented with glucose and yeast extract, the optimum pH for the growth of C. poinsettias was found to be 7.5. The organism requires thiamine, biotin, and pantothenic acid for growth. No absolute requirement was found for any amino acid, purine or pyrimidine although an amino acid mixture was stimulatory. Casamino acids could be substituted for the synthetic amino acid mixture. Yeast extract provided an additional factor(s) necessary for maximal growth. The results suggest that the unknown factor found in yeast extract might be purified by a combination of solvent extraction, and adsorption and elution from charcoal.
| Identifer | oai:union.ndltd.org:unt.edu/info:ark/67531/metadc504055 |
| Date | 12 1900 |
| Creators | Hooshdaran, Farideh |
| Contributors | Kester, Andrew S., Johansson, Karl R. |
| Publisher | North Texas State University |
| Source Sets | University of North Texas |
| Language | English |
| Detected Language | English |
| Type | Thesis or Dissertation |
| Format | iv, 23 leaves, Text |
| Rights | Public, Hooshdaran, Farideh, Copyright, Copyright is held by the author, unless otherwise noted. All rights reserved. |
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