Drinking water is a potential means for transmission of the opportunistic pathogen Legionella pneumophila. The objective of this research was to document the occurrence of L. pneumophila from source water, partially treated water after conventional water treatment, and distribution water. Water samples were examined for L. pneumophila by direct fluorescent antibody (DFA) techniques and by SYBR Green based real-time multiplex PCR. Primers were designed to amplify a 16S product of 490 bp and a mip product of 290 bp within L. pneumophila. Sensitivity of culture methods and PCR was determined by percent recovery and by using serial dilutions of positive control DNA, respectively. Eighty percent of source water samples were positive for L. pneumophila by real-time PCR versus 100% positive by DFA for L. pneumophila. Twenty percent of GAC filter water samples were positive for L. pneumophila by real-time PCR versus 40% positive by DFA. Distribution water samples yielded mixed results. Twenty percent of biofilms from the distribution system were positive for L. pneumophila by real-time PCR. This project confirms the potential for human infection by L. pneumophila from distribution water within the study area. / Department of Biology
| Identifer | oai:union.ndltd.org:BSU/oai:cardinalscholar.bsu.edu:handle/187986 |
| Date | January 2005 |
| Creators | Troxell, Stephen B. |
| Contributors | Warnes, Carl E. |
| Source Sets | Ball State University |
| Detected Language | English |
| Format | iii, 64 leaves : ill. (some col.) ; 28 cm. |
| Source | Virtual Press |
| Coverage | n-us-in |
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