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Development and application of a real time quantitative polymerase chain reaction assay for mitochondrial DNA.

Yu Man Him. / Thesis (M.Sc.)--Chinese University of Hong Kong, 2000. / Includes bibliographical references (leaves 45-50). / Abstracts in English and Chinese. / Abstract --- p.ii / Acknowledgement --- p.ii / Chapter 1. --- Introduction / The mitochondrion --- p.1 / The mitochondrial genome --- p.3 / Mitochondrial DNA and diseases --- p.7 / Circulating plasma DNA --- p.8 / Project aims --- p.8 / Chapter 2. --- Materials and Methods / Choice of gene quantification system --- p.9 / Real time quantitative PGR --- p.11 / 7700Sequence Detection System --- p.14 / The LightCycler ´ؤ an alternative fast analyzer --- p.15 / Quatntitation of starting copy number using real time PCR --- p.17 / Primer and probe design rules --- p.18 / Hot start PCR technique --- p.19 / Other anti-contamination measures --- p.20 / Test subjects --- p.21 / Sample processing --- p.23 / DNA extraction --- p.24 / Chapter 3. --- System Development / Choice of primer and probe sequences --- p.28 / Optimization of PCR conditions --- p.30 / Imprecision of TaqMan assays --- p.33 / MTRNR2 vs. MTCYB probes --- p.33 / Construction of standard curve --- p.35 / Chapter 4. --- Research Application / The trauma model --- p.37 / Plasma DNA as a marker for trauma severity --- p.37 / Results --- p.38 / Disussion --- p.41 / Chapter 5. --- Conclusion --- p.44 / Chapter 6. --- References --- p.45

Identiferoai:union.ndltd.org:cuhk.edu.hk/oai:cuhk-dr:cuhk_323020
Date January 2000
ContributorsYu, Man Him., Chinese University of Hong Kong Graduate School. Division of Chemical Pathology.
Source SetsThe Chinese University of Hong Kong
LanguageEnglish, Chinese
Detected LanguageEnglish
TypeText, bibliography
Formatprint, ii, 50 leaves : ill. ; 30 cm.
RightsUse of this resource is governed by the terms and conditions of the Creative Commons “Attribution-NonCommercial-NoDerivatives 4.0 International” License (http://creativecommons.org/licenses/by-nc-nd/4.0/)

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