Choi Yin Yee. / Thesis (M.Phil.)--Chinese University of Hong Kong, 2001. / Includes bibliographical references (leaves 164-187). / Abstracts in English and Chinese. / List of Abbreviations / List of Tables / List of Figures / Chapter Chapter 1: --- "Background of straw mushroom, Volvariella volvacea" / Chapter 1.1 --- History and economic value of straw mushroom --- p.1 / Chapter 1.2 --- Nutritional value and medical use of straw mushroom --- p.2 / Chapter 1.3 --- Cultivation of straw mushroom --- p.3 / Chapter 1.4 --- Life cycle and developmental stages of straw mushroom --- p.5 / Chapter 1.5 --- Objectives of the research --- p.6 / Chapter Chapter 2: --- Determination of straw mushroom quality during postharvest storage / Chapter 2.1 --- Introduction --- p.18 / Chapter 2.2 --- Materials and methods / Chapter 2.2.1 --- Determination of surface color changes --- p.19 / Chapter 2.2.2 --- Determination of weight loss and dry weight --- p.19 / Chapter 2.2.3 --- Examination of mushroom surface structure --- p.20 / Chapter 2.2.4 --- Statistical analysis --- p.20 / Chapter 2.3 --- Results and discussion / Chapter 2.3.1 --- Browning during postharvest storage --- p.20 / Chapter 2.3.2 --- Weight loss and dry weight changes during storage --- p.22 / Chapter 2.3.3 --- Surface structural changes during postharvest storage --- p.23 / Chapter Chapter 3: --- Physiological studies of straw mushroom during postharvest storage / Chapter 3.1 --- Introduction --- p.31 / Chapter 3.2 --- Materials and methods / Chapter 3.2.1 --- Materials --- p.33 / Chapter 3.2.2 --- Respiration rates of different produce --- p.34 / Chapter 3.2.3 --- Changes of respiration rate and detection of ethylene production in straw mushroom --- p.34 / Chapter 3.2.4 --- Exogenous ethylene treatment --- p.34 / Chapter 3.3 --- Results and discussion / Chapter 3.3.1 --- Comparative studies of respiration rates between different fresh produce --- p.35 / Chapter 3.3.2 --- Ethylene production and effect of exogenous ethylene effect on straw mushroom --- p.36 / Chapter 3.3.3 --- Change in respiration during postharvest storage --- p.37 / Chapter Chapter 4: --- Biochemical studies of straw mushroom during postharvest storage / Chapter 4.1 --- Introduction / Chapter 4.1.1 --- Hydro lytic enzymes / Chapter 4.1.1.1 --- Protease --- p.43 / Chapter 4.1.1.2 --- Lipase --- p.44 / Chapter 4.1.1.3 --- Chitinase --- p.46 / Chapter 4.1.2 --- Enzymatic browning / Chapter 4.1.2.1 --- Background --- p.48 / Chapter 4.1.2.2 --- Action of polyphenol oxidase --- p.49 / Chapter 4.1.2.3 --- Control of enzymatic browning --- p.50 / Chapter 4.2 --- Materials and methods / Chapter 4.2.1 --- Source --- p.52 / Chapter 4.2.2 --- Enzyme extraction --- p.52 / Chapter 4.2.3 --- Soluble protein determination --- p.52 / Chapter 4.2.4 --- Protease assay --- p.53 / Chapter 4.2.5 --- Lipase assay --- p.53 / Chapter 4.2.6 --- Chitinase assay --- p.54 / Chapter 4.2.7 --- PPO assay --- p.54 / Chapter 4.2.8 --- Optimal pH determination and substrate specificity of PPO --- p.55 / Chapter 4.2.9 --- PPO inhibitors studies --- p.56 / Chapter 4.2.10 --- Localization of PPO in the fruit body of straw mushroom --- p.56 / Chapter 4.3 --- Results and discussion / Chapter 4.3.1 --- Protease activity during postharvest storage --- p.57 / Chapter 4.3.2 --- Lipase activity during postharvest storage --- p.58 / Chapter 4.3.3 --- Chitinase activity during postharvest storage --- p.59 / Chapter 4.3.4 --- pH optimum and substrate specificity of PPO --- p.61 / Chapter 4.3.5 --- PPO activity during postharvest storage --- p.62 / Chapter 4.3.6 --- Effect of various inhibitors on PPO activity --- p.63 / Chapter 4.3.7 --- Localization of PPO in the fruit body of straw mushroom --- p.64 / Chapter Chapter 5: --- Microorganisms in the fruit body of straw mushroom during postharvest storage / Chapter 5.1 --- Introduction --- p.81 / Chapter 5.2 --- Materials and methods / Chapter 5.2.1 --- Evaluation of bacterial population --- p.85 / Chapter 5.2.2 --- Isolation of bacteria --- p.85 / Chapter 5.2.3 --- Treatment of antibacterial agents --- p.87 / Chapter 5.2.4 --- Effect of moisture on bacterial population --- p.87 / Chapter 5.2.5 --- Statistical analysis --- p.87 / Chapter 5.3 --- Results and discussion / Chapter 5.3.1 --- Bacterial population during postharvest storage --- p.88 / Chapter 5.3.2 --- Bacteria growth and mushroom deterioration --- p.89 / Chapter 5.3.3 --- Identification of bacteria isolated from straw mushroom --- p.92 / Chapter 5.3.4 --- Relationship between moisture and bacterial population --- p.95 / Chapter Chapter 6: --- Preservative studies of straw mushroom / Chapter 6.1 --- Introduction --- p.107 / Chapter 6.1.1 --- Chemicals treatment in produce --- p.109 / Chapter 6.1.1.1 --- Application of browning inhibitors --- p.110 / Chapter 6.1.1.2 --- Role of calcium in preservation --- p.111 / Chapter 6.1.2 --- Modified atmosphere technology --- p.114 / Chapter 6.2 --- Materials and methods / Chapter 6.2.1 --- Dipping experiment with browning inhibitors --- p.117 / Chapter 6.2.2 --- Application CaCl2 in straw mushroom cultivation --- p.117 / Chapter 6.2.3 --- Postharvest storage of straw mushroom under modified atmosphere --- p.118 / Chapter 6.2.4 --- Statistical analysis --- p.119 / Chapter 6.3 --- Results and discussion / Chapter 6.3.1 --- Effect of browning inhibitors on mushroom quality --- p.119 / Chapter 6.3.2 --- Effect of calcium chloride on mushroom quality --- p.121 / Chapter 6.3.3 --- Effect of modified atmosphere on mushroom quality --- p.124 / Chapter Chapter 7: --- Conclusion --- p.159 / References --- p.164
| Identifer | oai:union.ndltd.org:cuhk.edu.hk/oai:cuhk-dr:cuhk_323507 |
| Date | January 2001 |
| Contributors | Choi, Yin Yee., Chinese University of Hong Kong Graduate School. Division of Biology. |
| Source Sets | The Chinese University of Hong Kong |
| Language | English, Chinese |
| Detected Language | English |
| Type | Text, bibliography |
| Format | print, 187 leaves : ill. ; 30 cm. |
| Rights | Use of this resource is governed by the terms and conditions of the Creative Commons “Attribution-NonCommercial-NoDerivatives 4.0 International” License (http://creativecommons.org/licenses/by-nc-nd/4.0/) |
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