If the primary sequence of a protein is known, what is its three-dimensional structure?
This is one of the most challenging problems in molecular biology and has many applications
in proteomics. During the last three decades, this issue has been extensively researched.
Techniques such as the protein folding approach have been demonstrated to be promising in predicting the core areas of proteins - α-helices and β-strands. However, loops that contain no regular units of secondary structure elements remain the most difficult regions for prediction.
The protein loop prediction problem is to predict the spatial structure of a loop given the primary sequence of a protein and the spatial structures of all the other regions. There are two major approaches used to conduct loop prediction – the ab initio folding and database searching methods. The loop prediction accuracy is unsatisfactory because of the hypervariable property of the loops.
The key contribution proposed by this thesis is a novel fragment assembly algorithm using
branch-and-cut to tackle the loop prediction problem. We present various pruning rules to
reduce the search space and to speed up the finding of good loop candidates. The algorithm
has the advantages of the database-search approach and ensures that the predicted loops are physically reasonable. The algorithm also benefits from ab initio folding since it enumerates all the possible loops in the discrete approximation of the conformation space.
We implemented the proposed algorithm as a protein loop prediction tool named LoopLocker.
A test set from CASP6, the world wide protein structure prediction competition, was used to
evaluate the performance of LoopLocker. Experimental results showed that LoopLocker is
capable of predicting loops of 4, 8, 11-12, 13-15 residues with average RMSD errors of
0.452, 1.410, 1.741 and 1.895 A respectively. In the PDB, more than 90% loops are fewer
than 15 residues. This concludes that our fragment assembly algorithm is successful in
tackling the loop prediction problem.
| Identifer | oai:union.ndltd.org:WATERLOO/oai:uwspace.uwaterloo.ca:10012/2655 |
| Date | January 2006 |
| Creators | Liu, Zhifeng |
| Source Sets | University of Waterloo Electronic Theses Repository |
| Language | English |
| Detected Language | English |
| Type | Thesis or Dissertation |
| Format | 855611 bytes, application/pdf |
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