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Previous issue date: 2014-09-22 / This thesis had the purpose to evaluate the action of a new irrigant, QMix, on bacterial endotoxins as well as the effect of its combination with sodium hypochlorite on the root canal walls. Having the main purpose to evaluate its action on bacterial endotoxins, 50 extracted human teeth were contaminated in vitro with endotoxins and irrigated with QMix, EDTA, chlorhexidine (CHX) and sodium hypochlorite (NaOCl). It was used a Limulus Amebocyte Lysate (LAL) assay to quantify the remaining endotoxins after the irrigation. The results of this first study showed that QMix reduced the endotoxins level when compared to the other irrigant solutions that were tested. The second study from this thesis had the purpose to observe in scanning electron microscopy (SEM) the formation of precipitate on the root canal walls due to the concomitant use of NaOCl and QMix. The results from this study showed that despite the presence of CHX in the composition of QMix, its use after the chemical mechanical preparation of the canal with NaOCl did not result in the formation of a precipitate on the root canal walls. On the contrary, the images in SEM showed clean walls with exposed dentinal tubules. Once it is a new product there are still few studies about QMix. Although, according to the results presented on this study, it can be concluded that QMix is effective on the elimination of bacterial endotoxins from the root canal, not showing any precipitate formation when used concomitantly with NaOCl. / Este trabalho teve o objetivo de avaliar a a??o de um novo irrigante, o QMix, sobre endotoxinas bacterianas, bem como o efeito da sua combina??o com hipoclorito de s?dio sobre as paredes do canal radicular. Com a finalidade de avaliar sua a??o sobre endotoxinas bacterianas, 50 dentes humanos extra?dos foram contaminados in vitro com endotoxinas, e preenchidos com QMix, EDTA, clorexidina (CHX) e hipoclorito de s?dio (NaOCl). Foi utilizado um teste de Lisado de Ameb?cito de Limulus (LAL) para quantifica??o de endotoxinas remanescentes ap?s a irriga??o. Os resultados deste primeiro estudo demonstraram que o QMix reduziu os n?veis de endotoxinas quando comparado com as outras solu??es irrigadoras testadas. O segundo estudo da presente tese teve o prop?sito de observar em Microscopia Eletr?nica de Varredura (MEV) a forma??o de precipitado sobre as paredes do canal decorrente do uso concomitante de NaOCl e QMix. Os resultados deste estudo demonstraram que, apesar da presen?a de CHX na composi??o do QMix, seu uso ap?s o preparo qu?mico mec?nico do canal com NaOCl n?o resultou na forma??o de precipitado sobre as paredes do canal. Muito pelo contr?rio, as imagens em MEV apresentaram paredes limpas com t?bulos dentin?rios expostos. Por se tratar de um produto novo, ainda existem poucos estudos sobre o QMix. Por?m, de acordo com os resultados apresentados neste trabalho, pode-se concluir que o QMix ? eficaz na elimina??o de endotoxinas bacterianas do interior do canal radicular, e seu uso concomitante com NaOCl n?o resulta na forma??o de precipitado sobre as paredes do canal.
| Identifer | oai:union.ndltd.org:IBICT/oai:tede2.pucrs.br:tede/1273 |
| Date | 22 September 2014 |
| Creators | Gr?ndling, Grasiela Sabrina Longhi |
| Contributors | Figueiredo, Jos? Antonio Poli de |
| Publisher | Pontif?cia Universidade Cat?lica do Rio Grande do Sul, Programa de P?s-Gradua??o em Odontologia, PUCRS, BR, Faculdade de Odontologia |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/doctoralThesis |
| Format | application/pdf |
| Source | reponame:Biblioteca Digital de Teses e Dissertações da PUC_RS, instname:Pontifícia Universidade Católica do Rio Grande do Sul, instacron:PUC_RS |
| Rights | info:eu-repo/semantics/openAccess |
| Relation | -8096554818733665164, 500, 600, 4673435736271820140 |
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