Many studies describe the beneficial effect of probiotic bacteria of the gastrointestinal tract and their immunomodulatory effects. The aim of this diploma thesis was to evaluate the effect of probiotics, which are commonly used in human and animal nutrition, on the viability of the cells of the immune system. For the experiment were selected blood monocytes, which were isolated from porcine and human blood by dextran sedimentation. Monocyte population was co-cultivated in vitro with probiotic strains Bifidobacterium bifidum, Lactobacillus rhamnosus and Enterococcus faecium for 2 and 4 hours. The percentage of apoptosis and necrosis of monocytes was analysed by flow cytometry. The results showed statistically significant differences in the proportion of apoptotic and necrotic of porcine and human monocytes. It is obvious that the selected strains of probiotics have immunomodulatory effect on immune cells. Enterococcus faecium and Lactobacillus rhamnosus induce cell death of porcine and human monocytes during short-term cultivation in vitro.
| Identifer | oai:union.ndltd.org:nusl.cz/oai:invenio.nusl.cz:362765 |
| Date | January 2016 |
| Creators | Vejrychová, Šárka |
| Source Sets | Czech ETDs |
| Language | Czech |
| Detected Language | English |
| Type | info:eu-repo/semantics/masterThesis |
| Rights | info:eu-repo/semantics/restrictedAccess |
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