The central 5-hydroxytryptamine ( Serotonin, 5-HT ) system play an
important role in the brain sexual differentiation in tilapia,Oreochromis
mossambicus. ¡@The 5-HT1A receptor is involved in the neural develop-
ment of brain.¡@The cDNA sequences of 5-HT1A receptor of tilapia, Ore-
ochromis mossambicus have been cloned in our laboratory.¡@In the
present study, 1633 bp of DNA sequence of 5-HT1A receptor from the
transcription start site ( TSS ) were cloned by 5¡¦ rapid amplification of
cDNA end ( 5¡¦ RACE ) and Genome Walker DNA Walking.¡@The 1633
bp of DNA sequence divided into six different fragments to detect the
luciferase activity in the HeLa cell.¡@The bioinformatic analysis was
performed on the fragment which showed the highest luciferase activity
for predicting the transcription factor binding site.¡@These results showed
that there is a activator protein-1 ( AP-1 ) binding site in the fragment
form -52 bp to -46 bp.¡@The site-direct mutagenesis and electrophoresis
mobility shift assay was performed on the fragment form -52 bp to -46 bp
.¡@The present results indicate that there is a transcription factor binding
site in the fragment form -52 bp to -46 bp.¡@This transcription factor
binding site could be a AP-1 binding site.
| Identifer | oai:union.ndltd.org:NSYSU/oai:NSYSU:etd-0726105-131911 |
| Date | 26 July 2005 |
| Creators | shiun, Tsai-jia |
| Contributors | Li-Hsueh Wang, Ching-Lin Tasi, Yi-Ren Hong, Hsiao-Sheng Liu |
| Publisher | NSYSU |
| Source Sets | NSYSU Electronic Thesis and Dissertation Archive |
| Language | Cholon |
| Detected Language | English |
| Type | text |
| Format | application/pdf |
| Source | http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0726105-131911 |
| Rights | not_available, Copyright information available at source archive |
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