In this study, we determined the immunogenicity of CWP2 and its potential as a vaccine candidate against giardiasis. CWP2 was expressed as a recombinant protein with an hexa-histidine affinity tag and was isolated from inclusion bodies. When BALB/c mice were immunized with CWP2, a specific IgA was detected in the feces. When mice were immunized with CWP2 + cholera toxin, as an adjuvant, IgA in the feces, and IgA, IgG1, and IgG2a in the serum, all specific to CWP2, were detected. Also, CD-1 mice were infected with G. muris and presence of specific IgA antibodies to CWP2 were detected in the feces. This result indicated that CWP2 was recognized by the immune system in a natural infection. IL-4 and IL-5 were released from Peyer's patches (PP) and mesenteric lymph nodes (MLN) cells when stimulated with concanavalin A. In spleen cells, IFN-gamma, IL-4, and IL-5 were released when stimulated with concanavalin A. However, in PP, MLN and spleen cells, the levels of cytokines were barely detectable when stimulated with CWP2. The presence of IgG2a (Th1), IgA and IgG1 (Th2) as the production of IFN-gamma (Th1), IL-4 and IL-5 (Th2) confirmed that CWP2, when presented orally to mice, stimulates both a Th1 and Th2 type immune response, locally and systemically.
| Identifer | oai:union.ndltd.org:LACETR/oai:collectionscanada.gc.ca:QMM.30683 |
| Date | January 2000 |
| Creators | Larocque, Renée, 1975- |
| Contributors | Faubert, G. M. (advisor) |
| Publisher | McGill University |
| Source Sets | Library and Archives Canada ETDs Repository / Centre d'archives des thèses électroniques de Bibliothèque et Archives Canada |
| Language | English |
| Detected Language | English |
| Type | Electronic Thesis or Dissertation |
| Format | application/pdf |
| Coverage | Master of Science (Institute of Parasitology.) |
| Rights | All items in eScholarship@McGill are protected by copyright with all rights reserved unless otherwise indicated. |
| Relation | alephsysno: 001764186, proquestno: MQ64387, Theses scanned by UMI/ProQuest. |
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