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siqueira_ak_me_botfmvz.pdf: 464792 bytes, checksum: aae10b408540ec568f7000a9c01f5a54 (MD5) / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) / Escherichia coli e considerado o principal agente causal de infeccao de trato urinario (ITU) e piometra em caes. A patogenicidade das linhagens esta relacionada a presenca de adesinas e diferentes fatores de virulencia. Foram avaliadas alteracoes hematologicas e diferentes fatores de virulencia em 51 linhagens de E. coli isoladas de ITU, 52 de piometra e 55 de fezes de caes sem sinais entericos. A producao de ¿-hemolisina foi verificada em 26 (51,0%) das estirpes de ITU e em 20 (38,5%) de piometra. Exames hematologicos revelaram principalmente anemia, trombocitopenia e leucocitose por neutrofilia e monocitose nos caes com ITU e piometra. Os maiores indices de sensibilidade nas 158 estirpes foram observados para norfloxacina, ciprofloxacina e enrofloxacina em mais de 60% dos isolados. Os maiores indices de resistencia foram encontrados em 60% ou mais das estirpes com o uso de sulfametoxazole/trimetoprim. Linhagens resistentes a tres ou mais antimicrobianos foram constatadas em 24 (47,1%) de ITU, 7 (13,5%) de piometra e 4 (7,3%) das fezes, das quais respectivamente, 17 (33,3%), 1 (1,9%) e 3 (5,5%), com resistencia multipla a cinco ou mais drogas. fimH foi observado em mais de 90% dos isolados. papC foi detectado em 12 (23,5%) linhagens de ITU, 19 (36,5%) de piometra e 10 (18,2%) das fezes. papGI nao foi detectado, enquanto papGII foi observado em 3 (5,8%) isolados de piometra. papGIII foi expressado em 10 (19,6%) linhagens de ITU, 15 (28,8%) de piometra e 9 (16,4%) das fezes. sfaS foi encontrado em 22 (43,1%) de ITU, 24 (46,1%) de piometra e 19 (34,5%) das fezes. afa foi detectado em 1 (1,9%) linhagem de ITU e de piometra... / Escherichia coli is considered the more important microrganism in urinary tract infection (UTI) and pyometra in dogs. The pathogenicity of strains is associated with different adhesins and virulence factors. Haematological exams and different virulence factors was evaluated in 51 E. coli strains isolated from UTI, 52 from pyometra and 55 from feces of dogs without enteric signs. Alpha-haemolysin was verified in 26 (51.0%) strains from UTI and 20 (38.5%) from pyometra. Haematological exams revealed mainly anaemia, thrombocytopenia and leucocytosis by neutrophilia and monocitosis in dogs with UTI and pyometra. Norfloxacin, ciprofloxacin and enrofloxacin were the most-effective drugs (>60%) for 158 E. coli strains. High rates of E. coli resistance to antimicrobials were observed in 60% or more of strains using sulfametoxazole/trimetoprim. Multiple drug resistance for three or more antimicrobials was observed in 2 (47.1%) strains isolated from UTI, 7 (13.5%) from pyometra and 4 (7.3%) from feces. From these, 17 (33.3%), 1 (1.9%) and 3 (5.5%), respectively, showed multiple resistance to five or more drugs. fimH was observed in 90% or more of 158 isolates. papC was detected in 12 (23.5%) strains isolated from UTI, 19 (36.5%) from pyometra and 10 (18.2%) from feces. None strain expressed papGI, while papGII was observed in 3 (5.8%) strains of pyometra. papGIII was detected in 10(19.6%) strains of UTI, 15 (28.8%) from pyometra and 9 (16.4%) from feces. sfaS was observed in 22 (43.1%) strains of UTI, 24 (46.1%) of pyometra and 19 (34.5%) of feces. afa was identified in 1 (1.9%) strains isolated from UTI and pyometra...(Complete abstract, click electronic address below)
| Identifer | oai:union.ndltd.org:IBICT/oai:repositorio.unesp.br:11449/106031 |
| Date | 09 February 2007 |
| Creators | Siqueira, Amanda Keller [UNESP] |
| Contributors | Universidade Estadual Paulista (UNESP), Ribeiro, Márcio Garcia [UNESP] |
| Publisher | Universidade Estadual Paulista (UNESP) |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/doctoralThesis |
| Format | 136 f. |
| Source | Aleph, reponame:Repositório Institucional da UNESP, instname:Universidade Estadual Paulista, instacron:UNESP |
| Rights | info:eu-repo/semantics/openAccess |
| Relation | -1, -1 |
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