The thesis deals with molecular detection of resistance in populations of pollen beetle based on genetic polymorphisms in the VSSC gene of this pest. Population samples were collected in 2016-2018 in the Czech Republic, mainly in the Southwestern Bohemian Region. A total of 57 populations of pollen beetles with different degrees of resistance were analyzed. Nucleotide mutations of populations were monitored in two parts of the VSSC gene of pollen beetles - DIIS4 - S5 linker / DIIS5 and DIIIS6 gene, which is located in the VSSC binding domain. These parts were amplified and sequenced by the Sanger method. In the alignment of final sequences, mutations were searched. After that, the effect of these mutations on the amino acid sequence was determined and the effect on the resulting VSSC protein conformation was assessed.
| Identifer | oai:union.ndltd.org:nusl.cz/oai:invenio.nusl.cz:395231 |
| Date | January 2019 |
| Creators | KŘÍŽOVÁ, Lucie |
| Source Sets | Czech ETDs |
| Language | Czech |
| Detected Language | English |
| Type | info:eu-repo/semantics/masterThesis |
| Rights | info:eu-repo/semantics/restrictedAccess |
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