This thesis studies effect of cultivation conditions on the production of secondary me-tabolites in the green microalgae Scenedesmus quadricauda. During the experiment was studied influence of pH, UVC radiation, light intensity and various temperature conditions on the amount of biologically active compounds (polyphenols, flavonoids, antioxidants). Metabolites were determined using biochemical analytical methods. The total content of polyphenols was determined using the Folin-Ciocalteu method, which is based on chemical reaction of phenols with Folin-Ciocalteu reagent. The total con-tent of flavonoids was determined spectrophotometrically using AlCl3.6H2O with the addition of NaNO2 and 1 M NaOH. The total antioxidant capacity (TAC) was deter-mined using phosphomolybdenun antioxidant assay. Antioxidant activity was stimu-lated by UVC radiation. The highest value being recorded when samples were exposed to UVC for 5 minutes. Light intensity and the temperature changes did not have significant effect on antioxidant activity of studied samples. There was an increase in TAC in samples cultured in acid pH medium (4 and 5). The content of polyphenolic and flavonoid compounds with high light intensity (3500, 4000 lx) and high tempera-tures (35 °C, 40 °C) decreased. Conversely, changes in pH and UV-C stimulate biosyn-thesis of both polyphenols and flavonoids. The greatest increase of these compounds was observed in the medium with pH = 4 and the time of exposure to UV-C for 5 minutes a day.
| Identifer | oai:union.ndltd.org:nusl.cz/oai:invenio.nusl.cz:430220 |
| Date | January 2017 |
| Creators | Malyugina, Svetlana |
| Source Sets | Czech ETDs |
| Language | Czech |
| Detected Language | English |
| Type | info:eu-repo/semantics/masterThesis |
| Rights | info:eu-repo/semantics/restrictedAccess |
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