Return to search

Induction of interleukin-8 in lung epithelial cells by Gram negative bacteria and its modulation by pulmonary surfactant lipids

The surfactants did not inhibit IL-8 induced by IL-1[Special character omitted.] in A549 cells suggesting that they act at the level of the LPS receptor complex. Disruption of membrane micro-domains ('rafts') with methyl-[Special character omitted.]-cyclodextrin significantly inhibited bacteria and LPS induced IL-8 in A549 cells. Isolation of membrane raft containing fractions by sucrose density gradient ultra-centrifugation showed that TLR4 is recruited into membrane lipid rafts on cell stimulation with the bacteria or LPS. The surfactants inhibited the bacterial and LPS mediated translocation of TLR4 into raft domains suggesting that their mechanism of action involves inhibition of LPS receptor complex formation in lipid raft domains.

Identiferoai:union.ndltd.org:bl.uk/oai:ethos.bl.uk:583734
Date January 2006
CreatorsAlghaithy, Abdulaziz
PublisherCardiff University
Source SetsEthos UK
Detected LanguageEnglish
TypeElectronic Thesis or Dissertation
Sourcehttp://orca.cf.ac.uk/54104/

Page generated in 0.0018 seconds